Glutathione S-Transferase from equine liver
SIGMA/G6511 - lyophilized powder, ≥25 units/mg protein
Synonym: GST; Glutathione R-transferase; Glutathione S-alkenetransferase; Glutathione S-alkyltransferase; Glutathione S-aralkyltransferase; Glutathione S-aryltransferase; Glutathione S-epoxidetransferase
CAS Number: 50812-37-8
MDL Number: MFCD00131198
Product Type: Chemical
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| biological source | equine liver |
| composition | Protein, ≥60% |
| form | lyophilized powder |
| mol wt | 45-50 kDa |
| Quality Level | 200  |
| specific activity | ≥25 units/mg protein |
| storage temp. | −20°C |
| Analysis Note: | Protein determined by biuret. Purified and assayed by a modification of the method of Simons and Vander Jagt. Enzymatic activities are based on the conjugation of reduced glutathione with a second substrate. The individual proteins generally have activity with more than one class of substrate. |
| Biochem/physiol Actions: | Glutathione S-transferase (GST) from equine liver has been used- • as a constituent of Tris buffer for incubation of human umbilical vein endothelial cells (HUVEC) with atracurium to assess the proliferation of HUVEC in the presence of atracurium • as a component of GSB stock solution to determine GSB (glutathione S-bimane) conjugate fluorescence intensity in intact Arabidopsis cells • as an enzyme standard in spectrophotometric assay to determine the activity of GST |
| Biochem/physiol Actions: | Glutathione S-transferases are a family of proteins that catalyze the conjugation of reduced glutathione with a variety of hydrophobic chemicals containing electrophilic centers. |
| Biochem/physiol Actions: | Protein family catalyzing conjugation of reduced glutathione with various hydrophobic chemicals. |
| General description: | Glutathione S-transferase (GST) is a major detoxification enzyme, and exists as multiple cytoplasmic and membrane-bound isozymes. These isozymes differ in their catalytic activity, as well as in their non-catalytic binding properties. Cytoplasmic isoforms of GST are encoded by five genes, namely α, θ, μ, σ and π. α, μ and π are the most abundant forms in mammals. Membrane bound GST forms are encoded by a single gene. |
| Other Notes: | One unit will conjugate 1.0 μmole of 1-chloro-2,4-dinitrobenze |
| Packaging: | 1, 5, 10 mg in glass bottle |
| Packaging: | 25 mg in poly bottle |
| Physical form: | Lyophilized powder containing Tris, reduced glutathione and EDTA. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥25 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 2.5.1.18 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |