Anti-Growth Factor Independence-1 antibody, Mouse monoclonal
SIGMA/G6670 - clone 2.5D.17, purified from hybridoma cell culture
Synonym: Anti-GFI1; Anti-ZNF163
MDL Number: MFCD02263329
Product Type: Chemical
Western Blotting Whole extract of A549 Human lung carcinoma cells were separated on SDS-PAGE and probed with Anti-Growth Factor Independence-1 antibody, Mouse monoclonal, Clone: 2.5D.17 (Cat. No. G6670). The antibody was developed using 1:4,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A2304). Lanes 1. 1 μg/mL antibody 2. 2 μg/mL antibody 3. 4 μg/mL antibody 4. Negative Control
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2.5D.17, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~55 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| microarray: suitable | |
| western blot: 1-2 μg/mL using total cell extract of A549 cells | |
| UniProt accession no. | Q99684  |
| Analysis Note: | The antibody may also be used in EMSA (electrophoretic mobility shift analysis). |
| Application: | Monoclonal Anti-Growth Factor Independence-1 (GFI1) antibody may be used in: • immunoblotting • immunohistochemistry • EMSA (electrophoretic mobility shift analysis) |
| Biochem/physiol Actions: | Growth factor independence-1 (Gfi1) plays a key role in hematopoiesis along with Gfi1B proteins. Cytokines that regulate innate immunity induce Gfi1 expression. Deficiency of this protein causes thymic and peripherial T lymphopenia and severe abnormalities in pre-T-cell development in mice. Mutations in this gene cause defects in T lymphocyte function and development in humans. Gfi1 autoregulates its expression in T lymphocytes. |
| Biochem/physiol Actions: | The antibody epitope resides within the GFI1 SNAG transcriptional-repressio |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Growth Factor Independence-1 (GFI1) (mouse IgG1 isotype) is derived from the hybridoma 2.5D.17 produced by the fusion of mouse myeloma cells (SP2 cells) and splenocytes from BALB/c mice immunized with rat GFI1. Growth Factor Independence 1 (GFI1) is a zinc-finger transcriptional repressor, that is located on human chromosome 1p22. Gfi1 is highly expressed in thymus while Gfi1B is the predominant factor expressed in spleen, and both are expressed in bone marrow. It has a repression domain named SNAG at the N-terminal.† The protein also has a zinc finger DNA binding domain that is homologous to the domain in Gfi1B. Both bind to same consensus DNA sequence |
| Immunogen: | rat growth factor independence-1 (GFI1). |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |