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α-Glycerophosphate Dehydrogenase from rabbit muscle

SIGMA/G6751 - Type I, ammonium sulfate suspension, 100-300 units/mg protein

Synonym: sn-Glycerol-3-phosphate Dehydrogenase from rabbit muscle; sn-Glycerol-3-phosphate:NAD+ 2-oxidoreductase

CAS Number: 9075-65-4
EC Number: 232-982-3
MDL Number: MFCD00040474
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-G6751-200UN 200 units
 $76.30
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45-G6751-1KU 1000 units
 $147.00
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45-G6751-5KU 5000 units
 $508.00
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45-G6751-10KU 10000 units
 $817.00
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biological source rabbit muscle
color white
foreign activity Lactic dehydrogenase, pyruvate kinase, aldolase, and glyceraldehyde-3-phosphate dehydrogenase ≤0.01%
  Triosephosphate isomerase ≤0.02%
form ammonium sulfate suspension
Quality Level 200 
specific activity 100-300 units/mg protein
storage condition (Tightly closed)
storage temp. 2-8°C
technique(s) activity assay: suitable
type Type I
Analysis Note: Protein determined by biuret.
Application: α-Glycerophosphate Dehydrogenase from rabbit muscle has been used:
• in the reaction mixture to measure the glycerol kinase activity
• to demonstrate compartmentalized enzymatic reactions where NADH is involved
• in the reaction mixture assay for L-fuculose-1-phosphate aldolase
Biochem/physiol Actions: α-Glycerophosphate dehydrogenase catalyzes the oxidation of L-α-glycerophosphate (Glp) to form dihydroxyacetone phosphate (DHAP) and hydrogen peroxide (H2O2). This enzyme is associated with respiratory electron transport chain, glycolysis, and phospholipid metabolism.
General description: α-Glycerophosphate dehydrogenase is a membrane protein and a flavin-linked primary dehydrogenase.

Research area: Cell Signaling
Other Notes: One unit will convert 1.0 μmole of dihydroxyacetone phosphate to α-glycerophosphate per min at pH 7.4 at 25 °C.
Physical form: Crystalline suspension in 3.2 M (NH4)2SO4 and 0.1 g/L EDTA solution, pH 6.0
Preparation Note: Prepared by modification of the method of Fondy.
RIDADR NONH for all modes of transport
WGK Germany WGK 2
Flash Point(F) Not applicable
Flash Point(C) Not applicable
activity specific activity: 100-300 units/mg protein
Storage Temp. 2-8°C
Enzyme Commission (EC) Number 1.1.1.8   ( BRENDA  | IUBMB  )
UNSPSC 12352204

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