Anti-Green Fluorescent Protein (GFP), N-terminal antibody, Mouse monoclonal
SIGMA/G6795 - clone GSN24, purified from hybridoma cell culture
Synonym: Anti-GFP
Product Type: Chemical
Western Blotting GFP Recombinannt protein were separated on SDS-PAGE and probed with Anti-Green Fluorescent Protein (GFP), N-terminal antibody, Mouse monoclonal, Clone: GSN24 (Cat. No. G6795). The antibody was developed using 1:10,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917). Lanes 1. 9 μL/lane antibody 2. 6 μL/lane antibody 3. 3 μL/lane antibody 4. Negative Control
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | GSN24, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen 27 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| 2-8°C | |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1-2 μg/mL using GFP fusion proteins expressed in extracts of transfected cells |
| Application: | Anti-Green Fluorescent Protein (GFP), N-terminal antibody, Mouse monoclonal has been used in: western blotting, enzyme linked immunosorbent assay (ELISA), immunofluorescence labeling. |
| Biochem/physiol Actions: | Green Fluorescent Protein (GFP) is a unique tool in cellular and molecular biology research to monitor gene expression and protein localization in living cells. In the jellyfish, A. victoria, GFP transduces the excitation energy resulting from emission of blue light of the photoprotein aequorin and reemits it as green light. |
| Biochem/physiol Actions: | The antibody reacts specifically with GFP fusion proteins. |
| Disclaimer: | Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Green Fluorescent Protein (GFP), N-terminal antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma GSN24 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2–8 °C for up to one month. For extended storage, store at –20 °C in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Solutions at working dilution should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C; 2-8°C |
| UNSPSC | 12352203 |