Anti-G9a Methyltransferase antibody produced in rabbit
SIGMA/G6919 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-BAT8; Anti-G9a/NG36; Anti-NG36
MDL Number: MFCD06411814
Product Type: Chemical
Western Blotting JOURNAL CITATION: G9a-mediated methylation of ERα links the PHF20/MOF histone acetyltransferase complex to hormonal gene expression. By: Zhang, X., Peng, D., et al. in Nat Commun, 2016. PubMed ID: 26960573 Image collected and cropped by CiteAb from the following publication, (http://www.nature.com/doifinder/10.1038/ncomms10810), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: G9a-mediated methylation of ERα links the PHF20/MOF histone acetyltransferase complex to hormonal gene expression. By: Zhang, X., Peng, D., et al. in Nat Commun, 2016. PubMed ID: 26960573 Image collected and cropped by CiteAb from the following publication, (http://www.nature.com/doifinder/10.1038/ncomms10810), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: G9a-mediated methylation of ERα links the PHF20/MOF histone acetyltransferase complex to hormonal gene expression. By: Zhang, X., Peng, D., et al. in Nat Commun, 2016. PubMed ID: 26960573 Image collected and cropped by CiteAb from the following publication, (http://www.nature.com/doifinder/10.1038/ncomms10810), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting 293-T cells nuclear extract were separated on SDS-PAGE and probed with Anti-G9a Methyltransferase antibody produced in Rabbit (Cat. No. G6919). The antibody was developed using 1:20,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 1 μg/mL antibody 2. 2 μg/mL antibody 3. 1 μg/mL antibody + inhibition with immunizing peptide 4. Negative Control
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 1-2 μg using 293-T cell extracts |
| microarray: suitable | |
| western blot: 1-2 μg/mL using nuclear extracts of 293-T cells | |
| UniProt accession no. | Q96KQ7  |
| Application: | Anti-G9a Methyltransferase antibody produced in rabbit has been used in: • immunoblotting • immunoprecipitation • immunohistochemical analysis (IHC) |
| Biochem/physiol Actions: | In vivo, G9a is essential for early embryonic development and plays a dominant role in H3-K9 methylation of euchromatin. G9a was found to be a critical component of the CtBP and E2F-6 transcriptional repressor complexes. G9a was found to be the enzyme responsible for mono and dimethylation within silent euchromatin. |
| Biochem/physiol Actions: | G9a is a lysine-preferring histone methyltransferase (HMTase) containing a SET domain. It has 10 to 20-fold stronger in vitro HMTase activity towards histone H3-Lys9 (H3-K9) when compared to Suv39H1, another member of the same family of HMTases. It also methylates Lys27 of histone H3. Suv39 h1 methylates only lysine 9. It is capable of mono and dimethylation within silent euchromatin, whereas Suv39h1 trimethylates at pericentric heterochromatin. G9a is an important factor in the early embryonic development and plays a dominant role in H3-K9 methylation of euchromatin in vivo. It also forms a crucial component of the CtBP and E2F-6 transcriptional repressor complexes. |
| Biochem/physiol Actions: | The antibody recognizes the two isoforms of G9a methyltransferase (~180 kDa and 160 kDa). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | G9a (also known as HLA-B-associated transcript 8 (BAT8), G9a/NG36, or NG36) was originally characterized as a molecule encoded by a gene mapped in the class III region of the human major histocompatibility complex locus. G9a as well as EuHTM1 (Eu-HMTase) were found to be the components of the multimeric protein complex E2F-6. |
| Immunogen: | synthetic peptide corresponding to amino acids 1193-1210 of human G9a, conjugated to KLH via an N-terminal added lysine residue. This sequence differs from the mouse sequence by 3 amino acids. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |