Endo-β-galactosidase from Bacteroides fragilis
SIGMA/G6920 - recombinant, expressed in E. coli, ≥140 units/mg protein, buffered aqueous solution
Synonym: β-Galactosidase bacterial; Keratanase
MDL Number: MFCD00131433
Product Type: Chemical
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| conjugate | (Glucosaminoglycan) |
| form | buffered aqueous solution |
| mol wt | 32 kDa |
| Quality Level | 100  |
| recombinant | expressed in E. coli |
| specific activity | ≥140 units/mg protein |
| sterility | aseptically filled |
| storage temp. | 2-8°C |
| Application: | Endo-β-galactosidase was used in fractional protein isolation. It was used for deglycosylation in glycoproteomics of the endothelial secretome of human endothelial cells. |
| Biochem/physiol Actions: | Internal β(1-4) galactose linkages in unbranched, repeating poly-Nacetyllactosamine [GlcNAc β(1-3)Gal β (1-4)] structures are the preferred substrate. Sulfated structures such as keratan sulfate are also cleaved. Branching and/or fucosylation of the substrate may reduce or completely inhibit cleavage. Sulfation of C-6 on galactose will block cleavage. Oligosaccharides of the neolacto-group are cleaved at greatly reduced rates depending on the deviation from the preferred substrate. For example, Gal β(1-3)GlcNAc β(1-3) Gal β(1-4)Glc is cleaved at 5X10-5 the rate of keratan sulfate β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop. |
| Other Notes: | One unit will release 1.0 μmole of reducing sugar from bovine corneal keratan sulfate per minute at 37 °C, pH 5.8. |
| Packaging: | 0.5 unit in glass insert |
| Physical form: | Aseptically filled solution in 20 mM Tris-HCl, pH 7.5 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥140 units/mg protein |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.2.1.103 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |