β-Glucuronidase from Helix pomatia
SIGMA/G7017 - Type HP-2, aqueous solution, ≥100,000 units/mL
Synonym: β-D-Glucuronide glucuronosohydrolase
CAS Number: 9001-45-0
MDL Number: MFCD00130629
Product Type: Chemical
β-Glucuronidase is routinely used to hydrolyze glucuronide-conjugates of drug metabolites in order to optimize sample preparation prior to LC-MS, GC and ELISA analysis.
LC-MS analysis of codeine-glucuronide enzymatic hydrolysis with six different Sigma-produced β-glucuronidase preparations. The limpet-derived enzyme G8132 performed best with this substrate, with reaction completion within 3–4 hours at 60 °C at 1 unit per mL. Comparative data above was performed at 60 °C at 10 unit per mL.
LC-MS analysis of hydromorphone-glucuronide enzymatic hydrolysis with six different Sigma-produced β-glucuronidase preparations. Molluscan enzymes performed similarly. The reaction was complete within 3–4 hours at 60 °C at 1 unit per mL. The E. coli enzymes exhibited low specificity for this substrate.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: G7017-25ML
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: G7017-10ML
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: G7017-5ML
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: G7017-1ML
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: G7017-2ML
| biological source | Helix pomatia |
| form | aqueous solution |
| Quality Level | 300  |
| secondary activity | ≤7,500 units/mL sulfatase |
| shipped in | wet ice |
| specific activity | ≥100,000 units/mL |
| storage temp. | 2-8°C |
| type | Type HP-2 |
| Analysis Note: | Many β-glucuronidases derived from mollusks also contain sulfatase activity. |
| Application: | • Sample preparation for LC/MS through digestion of glucuronide conjugated compounds for biomarker and toxicology research. • Removal of glucuronide conjugates such as for the preparation of Baicalein. |
| Application: | New Technical Article Comparing Performance of Different Enzymes Learn more about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine |
| Biochem/physiol Actions: | Endogenous beta-glucuronidase is a lysosomal enzyme involved in digestion of glucuronide-modified catabolites. Microbial beta-glucuronidase found in microbiota can cause re-absorption of metabolites by hydrolysis of glucuronide conjugates of xenobiotic and endobiotic metabolites. |
| Biochem/physiol Actions: | Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis |
| Features and Benefits: | • pH optima 4.0 - 5.0. • Sulfatase pH optima 6.2 • Low Sulfatase activity (<7,500 units/mL). • Ammonium Sulfate suspension. • Mollusk-derived beta-glucuronidase. • Useful for acidic hydrolysis of glucuronides in low pH samples. |
| General description: | β-Glucuronidase Type H-2 from Helix pomatia is a crude solution of enzymes derived from the digestive juices of the Roman snail. β-glucuronidases derived from mollusks often contain sulfatase activity. Both activities are useful in the hydrolysis of metabolites for toxicology and biomarker analysis. For a broader look at our β-Glucuronidase product line and their uses see our page about Drug Metabolite Analysis |
| Other Notes: | One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay). |
| Other Notes: | Similar to G0876, but produced by Sigma. |
| Other Notes: | Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C. |
| Packaging: | 25 mL in glass bottle |
| Physical form: | Aqueous solution in ~1.0 M ammonium sulfate with 3 mM sodium azide as preservative. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H317 - H334 |
| Precautionary statements | P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313 |
| Hazard Codes | Xn |
| Risk Statements | 42/43 |
| Safety Statements | 23-36/37-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥100,000 units/mL; secondary activity: ≤7,500 units/mL sulfatase |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.2.1.31 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |