Glucose Oxidase from Aspergillus niger

SIGMA/G7141 - Type X-S, lyophilized powder, 100,000-250,000 units/g solid (without added oxygen)

Synonym: β-D-Glucose:oxygen 1-oxidoreductase; G.Od.; GOx

CAS Number: 9001-37-0
EC Number: 232-601-0
MDL Number: MFCD00063989
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-G7141-10KU	10000 units	$73.00 1/EA	Add To Favorites
45-G7141-50KU	50000 units	$151.00 1/EA	Add To Favorites
45-G7141-250KU	250000 units	$567.00 1/EA	Add To Favorites
45-G7141-1MU	1000000 units	$1630.00 1/EA	Add To Favorites
45-G7141-2.5MU	2500000 units	$3540.00 1/EA	Add To Favorites

Glucose quantitation: Glucose is oxidized to gluconic acid and hydrogen peroxide by glucose oxidase. Hydrogen peroxide reacts with o-dianisidine in the presence of peroxidase to form a colored product. Oxidized o-dianisidine reacts with sulfuric acid to form a more stable colored product.

This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: G7141-2.5MU

12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.

Properties
Descriptions
Safety Info.
Basic Data

application(s)	diagnostic assay manufacturing
composition	Protein, ≥65%
foreign activity	Catalase ≤5 units/mg protein
form	lyophilized powder
greener alternative category	Enabling ,
greener alternative product characteristics	Waste Prevention Design for Energy Efficiency Learn more about the Principles of Green Chemistry .
InChI	1S/C6H12O6/c7-1-2-3(8)4(9)5(10)6(11)12-2/h2-11H,1H2/t2-,3-,4+,5-,6-/m1/s1
InChI key	WQZGKKKJIJFFOK-VFUOTHLCSA-N
mol wt	160 kDa
Quality Level	200
SMILES string	O1[C@H]([C@@H]([C@H]([C@@H]([C@H]1CO)O)O)O)O
specific activity	100,000-250,000 units/g solid (without added oxygen)
storage temp.	−20°C
sustainability	Greener Alternative Product
type	Type X-S

Analysis Note:	May contain traces of amylase, maltase, glycogenase, invertase, and galactose oxidase.
Analysis Note:	Protein determined by biuret
Application:	Glucose Oxidase from Aspergillus niger has been used: • in the (glucose oxidase) GO reagent to measure the glucose content by the glucose oxidase (GO) method • to activate the human renal carcinoma cell line for constructing the oxidative stress model • to study its influence in the paste on the analytical performance of the bioelectrode
Application:	Glucose oxidase is widely used in the food and pharmaceutical industries as well as a major component of glucose biosensors.
Biochem/physiol Actions:	Glucose oxidase (GOX) oxidizes D-glucose to D-gluconolactone and hydrogen peroxide. Hydrogen peroxide, the catalytic product of GOX, serves as an anti-bacterial and anti-fungal agent. It is safe and can be used in several industrial applications like baking, dry egg powder production, wine production, gluconic acid production, etc. It possesses electrochemical activity, which makes it extremely important in glucose sensors and in fuel cell applications.
Biochem/physiol Actions:	Glucose oxidase catalyses the oxidation of β-d-glucose to d-glucono-β-lactone and hydrogen peroxide, with molecular oxygen as an electron acceptor.
General description:	Mass of glucose oxidase (GOX), a flavoprotein ranges from approximately 130 to 175 kDa. Some fungi and insects are capable of producing GOX.
General description:	Molecular Weight: 160 kDa (gel filtration) pI: 4.2 Extinction coefficient: E^1% = 16.7 (280 nm) Glucose oxidase from Aspergillus niger is a dimer consisting of 2 equal subunits with a molecular mass of 80 kDa each. Each subunit contains one flavin adenine dinulceotide moiety and one iron. The enzyme is a glycoprotein containing ~16% neutral sugar and 2% amino sugars. The enzyme also contains 3 cysteine residues and 8 potential sites for N-linked glycosylation. Glucose oxidase is capable of oxidizing D-aldohexoses, monodeoxy-D-glucoses, and methyl-D-glucoses at varying rates. The pH optimum for glucose oxidase is 5.5, while it has a broad activity range of pH 4-7. Glucose oxidase is specific for β-D-glucose with a K_M of 33-110 mM. Glucose oxidase does not require any activators, but it is inhibited by Ag⁺, Hg²⁺, Cu²⁺, phenylmercuric acetate, and p-chloromercuribenzoate. It is not inhibited by the nonmetallic SH reagents: N-ethylmaleimide, iodoacetate, and iodoacetamide. Glucose oxidase can be utilized in the enzymatic determination of D-glucose in solution. As glucose oxidase oxidizes β-D-glucose to D-gluconolactate and hydrogen peroxide, horseradish peroxidase is often used as the coupling enzyme for glucose determination. Although glucose oxidase is specific for β-D-glucose, solutions of D-glucose can be quantified as α-D-glucose will mutorotate to β-D-glucose as the β-D-glucose is consumed by the enzymatic reaction.
General description:	We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in fuel cell research. For more information see the article in biofiles .
Other Notes:	One unit will oxidize 1.0 μmole of β-D-glucose to D-gluconolactone and H₂O₂ per min at pH 5.1 at 35 °C, equivalent to an O₂ uptake of 22.4 μl per min. If the reaction mixture is saturated with oxygen, the activity may increase by up to 100%.
Packaging:	10000, 50000, 250000, 1000000, 2500000 units in poly bottle

Symbol	GHS08
Signal word	Danger
Hazard statements	H334
Precautionary statements	P261 - P284 - P304 + P340 + P312 - P501
Hazard Codes	Xn
Risk Statements	42
Safety Statements	22-45
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1

activity	specific activity: 100,000-250,000 units/g solid (without added oxygen)
Storage Temp.	−20°C
Enzyme Commission (EC) Number	1.1.3.4 ( BRENDA \| IUBMB )
UNSPSC	12352204

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