Anti-GM130 (C-terminal) antibody produced in rabbit
SIGMA/G7295 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-GM130 (Golgi Matrix Protein of 130 kDa); Anti-Golgi Autoantigen; Anti-Golgi Matrix Protein GM 130
MDL Number: MFCD01633397
Product Type: Chemical
Immunocytochemistry-immunoflourescence JOURNAL CITATION: Itm2a expression in the developing mouse first lower molar, and the subcellular localization of Itm2a in mouse dental epithelial cells. By: Kihara, M., Kiyoshima, T., et al. in PLoS One, 2014. PubMed ID: 25079563 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0103928), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunofluorescence NRK normal Rat kidney cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 2 μg/mL Anti-GM130 (C-terminal) antibody produced in Rabbit (Cat. No. G7295). The antibody was developed using Goat Anti-Rabbit IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Western Blotting Whole extract of NRK Rat kidney cells were separated on SDS-PAGE and probed with Anti-GM130 (C-terminal) antibody produced in Rabbit (Cat. No. G7295). The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 0.1 μg/mL antibody 2. 0.2 μg/mL antibody 3. Negative Control
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~130 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 2-4 μg using Hela and 3T3 cell extracts. |
| indirect immunofluorescence: 0.2-0.4 μg/mL using rat NRK cells | |
| western blot (chemiluminescent): 0.1-0.2 μg/mL using whole extract of rat NRK cells | |
| UniProt accession no. | Q08379  |
| Application: | Anti-GM130 (C-terminal) antibody can be used in immunoblotting (approx. 130 kDa), indirect immunofluorescence (0.2-0.4 μg/mL using rat NRK cells), and immunoprecipitation (2-4 μg using Hela and 3T3 cell extracts). The antibody can also be used for chemiluminescent western blot (0.1-0.2 μg/mL using whole extract of rat NRK cells). |
| Application: | Anti-GM130 (C-terminal) antibody produced in rabbit has been used in: • immunoblotting • indirect immunofluorescence • immunoprecipitation • immunohistochemistry |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunohistochemistry (1 paper) Western Blotting (1 paper) |
| Biochem/physiol Actions: | Golgi Matrix Protein of 130 kDa (GM130) is involved in maintaining the structural integrity of the Golgi apparatus and vesicular transport. GM130 also interacts with Golgi reassembly-stacking protein of 65 kDa (GRASP65) and participated in postmitotic reassembly and stacking of the Golgi cisternae. The protein along with p115 and GRASP65 might acts as a template in nucleating the formation of the Golgi apparatus. GM130 in complex with GRASP65 and other proteins interacts with activated Rab1, a protein known to regulate the transport of newly synthesized proteins from the endoplasmic reticulum (ER) to the Golgi apparatus. This antibody can be used as a cis-Golgi network marker. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Golgi Matrix Protein of 130 kDa (GM130) is a peripheral cytoplasmic protein and is a member of the golgin family of Golgi autoantigens. GM130 is an extended rod-like protein containing coiled-coil domains and is tightly bound to Golgi membranes. |
| Immunogen: | synthetic peptide corresponding to the C-terminal region of human GM130 with an N-terminal added lysine, conjugated to KLH. The corresponding sequence is identical in rat and mouse GM130. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7,.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |