Anti-Rabbit IgG (whole molecule)–Gold antibody produced in goat
SIGMA/G7402 - affinity isolated antibody, aqueous glycerol suspension, 10 nm (colloidal gold)
MDL Number: MFCD00162787; MFCD00162787
Product Type: Chemical
Immunohistology
Paraformaldahyde-fixed adrenal tumor stained with Rabbit Anti-Human Chromogranin A followed by Goat Anti-Rabbit IgG, 10 nm Gold conjugate (Cat. No. G7402). Post reacted with uranyl acetate and lead citrate. Magnification 37,000x.
From K. Connolly, Dartmouth Electron Microscope Facility, Hanover, NH.
Paraformaldahyde-fixed adrenal tumor stained with Rabbit Anti-Human Chromogranin A followed by Goat Anti-Rabbit IgG, 10 nm Gold conjugate (Cat. No. G7402). Post reacted with uranyl acetate and lead citrate. Magnification 37,000x.
From K. Connolly, Dartmouth Electron Microscope Facility, Hanover, NH.
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | gold conjugate |
| form | aqueous glycerol suspension |
| particle size | 10 nm (colloidal gold) |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | dot blot: suitable |
| western blot: suitable |
| Application: | Anti-rabbit IgG (whole molecule)-gold antibody (diluted 1: 15) can be used in immunoelectron microscopy. It can also be used in western blotting. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immuno-electron microscopy (1 paper) Immunohistochemistry (1 paper) |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | IgG antibody plays a crucial role in humoral immune responses such as complement activation, phagocytosis, placental transport and cell surface-receptor binding. Anti-rabbit IgG (whole molecule)-gold antibody can be used as secondary antibody (diluted 1:50 in 0.01 M PBS containing 1% BSA and 5% NGS) in EM immunolabeling of GABA and glutamate by postembedding procedure. It can also be used in a modified dot blot assay to determine binding activity. Goat anti-rabbit IgG (whole molecule)-gold antibody reacts specifically with all goat immunoglobulins. |
| General description: | Immunoglobulins (Igs) are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs can be useful tools for the analysis of target proteins Anti-Rabbit IgG (whole molecule)-Gold antibody is specific for rabbit IgG. |
| Immunogen: | Rabbit IgG purified from normal rabbit serum. |
| Packaging: | 0.4 mL in glass insert |
| Packaging: | 2 mL in glass bottle |
| Physical form: | Colloidal suspension in Tris buffered saline, pH 8.2, with 30% glycerol (v/v), 1% bovine serum albumin (w/v), and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |