GABase from Pseudomonas fluorescens
SIGMA/G7509 - lyophilized powder, Protein ≥30 % by biuret
Synonym: GABase Enzyme; GABase from Pseudomonas; Pseudomonas GABase
MDL Number: MFCD00131113
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: G7509-25UN
| assay | ≥30 % (biuret) |
| biological source | Pseudomonas fluorescens |
| composition | Protein, ≥30% biuret |
| form | lyophilized powder |
| Quality Level | 200  |
| specific activity | ≥0.5 units/mg protein |
| storage temp. | −20°C |
| technique(s) | inhibition assay: suitable |
| Application: | GABase from Pseudomonas fluorescens has been used for GABase assay to quantify the extracellular and intracellular concentration of GABA (γ-aminobutyric acid). |
| Biochem/physiol Actions: | GABase catalyzes the conversion of γ-aminobutyric acid (GABA) to succinic acid in the central nervous system. It is used to measure GABA levels in various biological samples. p-Hydroxybenzaldehyde (HBA) is known to be a potential inhibitor on GABase. |
| General description: | Research area: Neuroscience GABase is a commercially available mixture composed of γ-aminobutyric acid aminotransferase (GABGT) and succinic semialdehyde dehydrogenase (SSDH) obtained from Pseudomonas fluorescens. |
| Other Notes: | One unit will convert 1.0 μmole of γ-aminobutyric acid (GABA) to succinic semialdehyde and then to succinate per min with a stoichiometric reduction of 1.0 μmole of NADP+ at pH 8.6 at 25 °C. |
| Packaging: | 10, 25 units in poly bottle |
| Physical form: | Partially purified lyophilized powder containing buffer salts and stabilizer |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥30 % (biuret) |
| activity | specific activity: ≥0.5 units/mg protein |
| Storage Temp. | −20°C |
| UNSPSC | 12352204 |