β-Glucuronidase from Escherichia coli
SIGMA/G7646 - Type VII-A, lyophilized powder, 5,000,000-20,000,000 units/g protein, pH 6.8 (30 min assay)
Synonym: β-D-Glucuronide glucuronosohydrolase
CAS Number: 9001-45-0
EC Number: 232-606-8
MDL Number: MFCD00199013
Product Type: Chemical
β-Glucuronidase is routinely used to hydrolyze glucuronide-conjugates of drug metabolites in order to optimize sample preparation prior to LC-MS, GC and ELISA analysis.
| composition | Protein, ~25% biuret |
| form | lyophilized powder |
| mol wt | 290 kDa |
| Quality Level | 300  |
| specific activity | 5,000,000-20,000,000 unit |
| storage temp. | −20°C |
| type | Type VII-A |
| Analysis Note: | Contains buffer salts and stabilizer. |
| Application: | New Technical Article Comparing Performance of Different Enzymes Learn more about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine |
| Application: | Effective in the hydrolysis of steroid glucuronides. |
| Application: | Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis |
| Other Notes: | One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at the pH 6.8 (30 min assay). |
| Packaging: | 100000, 500000 units in poly bottle |
| Packaging: | 5000, 25000 units in glass bottle |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H317 - H334 |
| Precautionary statements | P261 - P280 - P342 + P311 |
| Hazard Codes | Xn |
| Risk Statements | 42/43 |
| Safety Statements | 22-36/37-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 5,000,000-20,000,000 unit |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.2.1.31 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |