β-Glucuronidase from Helix pomatia
SIGMA/G7770 - Type HP-2S, aqueous solution, ≥90,000 units/mL
Synonym: β-D-Glucuronide glucuronosohydrolase
CAS Number: 9001-45-0
MDL Number: MFCD00130629
Product Type: Chemical
β-Glucuronidase is routinely used to hydrolyze glucuronide-conjugates of drug metabolites in order to optimize sample preparation prior to LC-MS, GC and ELISA analysis.
LC-MS analysis of codeine-glucuronide enzymatic hydrolysis with six different Sigma-produced β-glucuronidase preparations. The limpet-derived enzyme G8132 performed best with this substrate, with reaction completion within 3–4 hours at 60 °C at 1 unit per mL. Comparative data above was performed at 60 °C at 10 unit per mL.
LC-MS analysis of hydromorphone-glucuronide enzymatic hydrolysis with six different Sigma-produced β-glucuronidase preparations. Molluscan enzymes performed similarly. The reaction was complete within 3–4 hours at 60 °C at 1 unit per mL. The E. coli enzymes exhibited low specificity for this substrate.
| form | aqueous solution |
| Quality Level | 200  |
| secondary activity | ≤7,500 units/mL sulfatase |
| specific activity | ≥90,000 units/mL |
| sterility | sterile-filtered |
| storage temp. | 2-8°C |
| type | Type HP-2S |
| Analysis Note: | Many β-glucuronidases derived from mollusks also contain sulfatase activity. |
| Application: | New Technical Article Comparing Performance of Different Enzymes Learn more about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine |
| Application: | β-Glucuronidase from Helix pomatia has been used: • in the treatment of heparinized plasma sample for the hydrolysis of glucuronic acid-conjugated metabolites • in the pre-treatment of plasma sample for the determination of 1′-hydroxymidazolam (1′-OHMDZ) • for the treatment of bile samples from rainbow trouts for the hydrolysis of glucuronate and sulfate metabolites • in treating the cells from the sporulation media for the complete digestion of the cell walls of asci |
| Application: | β-glucuronidase was used in enzymic hydrolysis of tissue homogenates for liquid chromatography-electrospr |
| Biochem/physiol Actions: | β-glucuronidase (β-GIc) is an exoglycosidase that catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption. |
| Biochem/physiol Actions: | Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis |
| General description: | β-Glucuronidase Type H-2 from Helix pomatia is a crude solution of enzymes derived from the Roman snail. Many β-glucuronidases derived from mollusks also contain sulfatase activity. |
| Other Notes: | One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay). |
| Other Notes: | Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C. |
| Physical form: | Aqueous solution in ~1.0 M ammonium sulfate with 3 mM sodium azide as preservative. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H317 - H334 |
| Precautionary statements | P261 - P272 - P280 - P284 - P302 + P352 - P304 + P340 + P312 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| activity | specific activity: ≥90,000 units/mL; secondary activity: ≤7,500 units/mL sulfatase |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.2.1.31 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |