Galactose Oxidase from Dactylium dendroides
SIGMA/G7907 - ≥30 units/mg solid
Synonym: D-Galactose:oxygen 6-oxidoreductase
CAS Number: 9028-79-9
EC Number: 232-843-7
MDL Number: MFCD00131118
Product Type: Chemical
Galactose oxidase catalyzes the coversion of D-galactose to D-galacto-hexodialdose. Stoichiometry: D-Galactose + Oxygen → D-galacto-Hexodialdose + Hydrogen peroxide
| biological source | fungus (Dactylium dendroides) |
| form | lyophilized |
| Quality Level | 200  |
| specific activity | ≥30 units/mg solid |
| storage temp. | −20°C |
| Application: | Galactose Oxidase from Dactylium dendroides has been used as a component for galactose oxidase treatment of arabinogalactan. It has also been used to co-immobilise with peroxidase for the preparation of a biosensor for galactose detection. |
| Application: | Galactose oxidase may be used as an analytical tool for the specific determination of D-galactose in blood plasma, plant extracts, and phospholipids. It could be used for the characterization of terminal D-galactoside units in several polymers. It may also be useful in the determination of lactose. |
| Biochem/physiol Actions: | Galactose oxidase catalyzes the coversion of D-galactose to D-galacto-hexodialdose. 2-Deoxy- Essentially no oxidation of |
| Biochem/physiol Actions: | Galactose oxidase has several applications in bioanalytics and histology. This free radical enzyme possess high substrate specificity. |
| Biochem/physiol Actions: | The specificity for galactose and other sugars is similar to that described by Avigad. |
| General description: | Galactose oxidase is a member of radicalcoupled copper oxidases family. It is a fungal secretory enzyme. |
| General description: | Galactose oxidase is an extracellular copper-containing enzyme, secreted by the deuteromycete fungus Dactylium dendroides. It catalyzes the oxidation of a range of primary alcohols, including D-galactose, to the corresponding aldehyde, with reduction of oxygen to hydrogen peroxide. |
| Other Notes: | One unit will produce a ΔA425 of 1.0 per min at pH 6.0 at 25 °C, in a peroxidase and o-tolidine system. Reaction volume = 3.4 mL. Light path = 1 cm. |
| Physical form: | Lyophilized, contains buffer salts and stabilizer |
| Preparation Note: | Chromatographically purified |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥30 units/mg solid |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 1.1.3.9 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |