Monoclonal Anti-β-Galactosidase antibody produced in mouse
SIGMA/G8021 - clone GAL-13, ascites fluid
Synonym: Monoclonal Anti-β-Galactosidase
Product Type: Chemical
ELISA 1:1,000 Monoclonal Anti-Human IgG (γ-chain specific) antibody produced in Mouse, Clone: GG-5 (Cat. No. I5885), followed by 40 μg/mL Anti-Mouse IgG (Fab specific) antibody produced in Goat (Cat. No. M6898) and by serially diluted Monoclonal Anti-β-Galactosidase antibody produced in Mouse, Clone: GAL-13 (Cat. No. G8021) was used to detect Human IgG by indirect ELISA.
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | GAL-13, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | bacteria |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | dot blot: suitable using soluble β-galactosidase |
| immunocytochemistry: suitable | |
| indirect ELISA: 1:2,000 using mouse primary antibody, bridging antibody and Escherichia coli β-galactosidase |
| Application: | Monoclonal Anti-β-Galactosidase antibody produced in mouse has been used in: • immunostaining and histochemistry • immunostaining and microscopy • immunohistochemistry • immunofluorescence staining • enzyme linked immunosorbent assay(ELISA) • immuno dot blot |
| Biochem/physiol Actions: | β −galactosidase substrate is lactose. Synthetic substrates like o-nitrophenyl-β-d-galactopyranoside (oNPG) or p-nitrophenyl-β-d-galactopyranoside (pNPG) are used in enzymatic assays. It is also used as marker in studying gene expression. The use of monoclonal β -galactosidase anti- β -galactosidase (BGABG) soluble complex in the unlabeled antibody-enzyme method results in an intense signal with a very low background. |
| Biochem/physiol Actions: | The antibody may be used for amplification in immunoenzymatic staining by preparing a β-galactosidase anti-β-galactosidase (BGABG) soluble complex. It is not recommended for immunoblotting; it does not recognize denatured or reduced β-galactosidase. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-β-Galactosidase (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. β −galactosidase is a E coli protein and is a tetramer with 1023 amino acids. |
| Immunogen: | β-galactosidase from E. coli |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |