Anti-phospho-G3BP (pSer149) antibody produced in rabbit
SIGMA/G8046 - ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-G3BP1; Anti-GAP SH3 domain-binding protein; Anti-HDH-VIII; Anti-
MDL Number: MFCD02262927
Product Type: Chemical
Immunoblotting Whole extract of HeLa cells was separated on SDS-PAGE and probed with Rabbit Anti-phospho-G3BP (pSer149) (Cat. No. G8046). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 5 μg/mL antibody 2. 10 μg/mL antibody 3. 10 μg/mL antibody + G3BP non-phosphorylated-peptide (human, 139-151) 4. 10 μg/mL antibody + G3BP phosphorylated-peptide (human, 139-151 (pSer149))
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~68 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, rat (predicted), mouse (predicted) |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pSer149) |
| technique(s) | western blot: 5-10 μg/mL using whole extract of human K562 and HeLa cells |
| UniProt accession no. | Q13283  |
| Application: | Anti-phospho-G3BP (pSer149) antibody produced in rabbit is suitable for western blotting at a working concentration of 5-10μg/mL using whole extract of human K562 and HeLa cells. |
| Biochem/physiol Actions: | G3BP (Ras-GTPase-activating protein SH3 domain binding protein 1) exclusively cleaves between cytosine and adenine (CA). In dividing cells, G3BP interacts with RasGAP, linking between a RasGAP-mediated signaling pathway and RNA turnover. It is a RNA-binding protein that contains a carboxyl-terminal RNA binding domain, the RRM-type domain. It also has an amino-terminal domain homologous to nuclear transporter factor 2 (NTF2), and a central domain rich in acidic residues. The RRM domain is involved in the binding of G3BP to specific RNA sequences. A Ser149 residue is present 20 amino acids C terminal to the NTF2-like domain. Phosphorylation of this residue is crucial in mediating protein-protein interactions and in controlling the subcellular localization of G3BP. G3BP facilitates the assembly of stress granules that are invoved in the regulation of mRNA metabolism during stress. Dephosphorylation of Ser149 results in oligomerization and SG assembly. Overexpression of G3BP is observed in malignant tumors such as lung cancer, colon cancer, gastric cancer, and breast cancer. The level of G3BP expression in breast cancer specimens is positively correlated to the presence of lymph node metastasis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | G3BP (pSer149) encodes one of the DNA-unwinding enzymes which prefers partially unwound 3′-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. |
| General description: | G3BP (Ras-GTPase-activating protein SH3 domain binding protein 1) is a 68kDa, single-strand-specific endoribonuclease that is phosphorylation-dependent |
| Physical form: | Solution 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |