Glutamic-Pyruvic Transaminase from porcine heart
SIGMA/G8255 - lyophilized powder, ≥75 units/mg protein
Synonym: L-Alanine: 2-Oxoglutarate Aminotransferase; Alanine Aminotransferase (catalytic concentration); GPT
CAS Number: 9000-86-6
EC Number: 232-561-4
MDL Number: MFCD28970979
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: G8255-1KU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: G8255-2KU
| biological source | Porcine heart |
| color | light yellow to yellow |
| composition | Protein, 75-95% |
| foreign activity | |
| glutamic-oxalacetic transaminase ≤0.1% | |
| form | lyophilized powder |
| Quality Level | 200  |
| specific activity | ≥75 units/mg protein |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| Analysis Note: | Protein determined by biuret |
| Application: | Glutamic-Pyruvic Transaminase from porcine heart has been used: • in the transamination assay with a racemic mixture of synthetic 3-methyl-2-oxoglutarate • to remove glutamate from the cell culture medium used for culturing human embryonic kidney (HEK293) cells • in the lactate-dehydrogenase (LD)-coupled alanine transaminase (ALT) reaction kinetic studies |
| Biochem/physiol Actions: | Glutamic-pyruvic transaminase (GPT) is located in most cells in the body, but is found in highest concentrations in the liver. It is used as a marker of liver disease due to the release of GPT into the blood following liver cell necrosis. |
| Biochem/physiol Actions: | Glutamic-pyruvic transaminase (GPT) mediates the transfer of the amino group to α-ketoglutarate from l-alanine which results in pyruvate and glutamate.Thereby GPT plays a key role in gluconeogenesis and amino acid metabolism. The role of GPT in various kinds of tumors has been reported. |
| General description: | Glutamic-pyruvic transaminase (GPT) is localized majorly in the liver. Research area: Cell Signaling |
| Other Notes: | One unit will convert 1.0 μmole of α-ketoglutarate to |
| Packaging: | 1000, 2000 units in poly bottle |
| Packaging: | 200 units in glass bottle |
| Physical form: | Lyophilized powder containing citrate and acetate |
| Preparation Note: | Tightly closed. Dry. Keep locked up or in an area accessible only to qualified or authorized |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥75 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 2.6.1.2 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |