Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides
SIGMA/G8404 - recombinant, expressed in E. coli, ammonium sulfate suspension, ≥550 units/mg protein (biuret)
Synonym: Entner-Doudoroff enzyme; G6PD; G6PDH; NADP glucose 6-phosphate dehydrogenase; G-6-P-DH
CAS Number: 9001-40-5
MDL Number: MFCD00081656
Product Type: Chemical
Mechanism of Glucose-6-phosphate Dehydrogenase D-glucose 6-phosphate + NADP+ +H2O→ 6-phospho-D-gluconate + NADPH + H+
| biological source | bacterial (Leuconostoc mesenteroides) |
| foreign activity | creatine phosphokinase, glutathione reductase, myokinase, NADH oxidase, NADPH oxidase, phosphoglucomutase, 6-phosphogluconic dehydrogenase, phosphoglucose isomerase, lactic dehydrogenase, hexokinase ≤0.01% |
| form | ammonium sulfate suspension |
| mol wt | 128 kDa |
| Quality Level | 300  |
| recombinant | expressed in E. coli |
| specific activity | ≥550 units/mg protein (biuret) |
| storage condition | (Tightly closed) |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | UPI0021A4D550  |
| Application: | Glucose-6-phosphate dehydrogenase has been used: • to test ketose reductase activity in developing maize endosperm. • to determine the levels of mannose in coronary heart disease patient-derived serum • to study its activity on extracellular polymeric substance (EPS) extract to determine cell lysis through sonication • to determine the glucose uptake in cultured human muscle satellite cells |
| Biochem/physiol Actions: | G6PD provides all cells with reducing power as nicotinamide adenine dinucleotide phosphate (NADPH) which enables the cells to balance oxidative stress. Mutations in the G6PD gene are associated with X-linked G6PD deficiency, a hereditary genetic defect such as neonatal jaundice and acute hemolytic anemia. |
| Biochem/physiol Actions: | Glucose-6-phosphate dehydrogenase (G6PD) catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway. |
| General description: | Glucose 6-phosphate dehydrogenase (G-6-P-DH) is a key regulatory enzyme in the first step of the pentose phosphate pathway. G-6-P-DH is a glycoprotein with a molecular mass of 128 kDa (gel filtration).G6PD is a dimer and consists of a single active site at each subunit. Research area: Cell Signaling |
| Other Notes: | One unit will oxidize 1.0 μmole of |
| Other Notes: | The volume is lot specific and can be calculated using the data: For example, lot 0000114274 Units per mg Protein = 846 Mg Protein per mL - 9 Units per mL = 7614 G8404-1000U = approx 132 ul |
| Physical form: | Suspension in 3.2 M (NH4)2SO4 containing 50 mM Tris and 1 mM MgCl2, pH 7.5 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥550 units/mg protein (biuret) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 1.1.1.49 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |