β-Glucuronidase from Escherichia coli
SIGMA/G8420 - ≥20,000 units/mg protein, recombinant, expressed in E. coli overproducing strain, lyophilized powder
Synonym: β-D-Glucuronide glucuronosohydrolase
CAS Number: 9001-45-0
EC Number: 232-606-8
MDL Number: MFCD00199013
Product Type: Chemical
β-Glucuronidase is routinely used to hydrolyze glucuronide-conjugates of drug metabolites in order to optimize sample preparation prior to LC-MS, GC and ELISA analysis.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: G8420-100KU
| composition | , ~25% |
| form | lyophilized powder |
| mol wt | 290 kDa |
| Quality Level | 300  |
| recombinant | expressed in E. coli overproducing strain |
| specific activity | ≥20,000 units/mg protein |
| storage temp. | −20°C |
| Analysis Note: | Contains buffer salts and stabilizer. |
| Application: | New Technical Article Comparing Performance of Different Enzymes Learn more about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine |
| Application: | Effective in the hydrolysis of steroid glucuronides. |
| Application: | Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis |
| Other Notes: | One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at the pH 6.8 (30 min assay). |
| Packaging: | 25000, 100000 units in glass bottle |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H317 - H334 |
| Precautionary statements | P261 - P280 - P342 + P311 |
| Hazard Codes | Xn |
| Risk Statements | 42/43 |
| Safety Statements | 22-36/37-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥20,000 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.2.1.31 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |