Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides
SIGMA/G8529 - recombinant, expressed in E. coli, lyophilized powder, ≥550 units/mg protein (biuret)
Synonym: G-6-P-DH
CAS Number: 9001-40-5
EC Number: 232-602-6
MDL Number: MFCD00081656
Product Type: Chemical
Mechanism of Glucose-6-phosphate Dehydrogenase D-glucose 6-phosphate + NADP+ +H2O→ 6-phospho-D-gluconate + NADPH + H+
| application(s) | agriculture |
| composition | Protein, 10-40% biuret |
| foreign activity | creatine phosphokinase, glutathione reductase, myokinase, NADH oxidase, NADPH oxidase, phosphoglucomutase, 6-phosphogluconic dehydrogenase, phosphoglucose isomerase, lactic dehydrogenase, hexokinase ≤0.01% |
| form | lyophilized powder |
| Quality Level | 300  |
| recombinant | expressed in E. coli |
| specific activity | ≥550 units/mg protein (biuret) |
| storage temp. | 2-8°C |
| Application: | Glucose-6-phosphate dehydrogenase from Leuconostoc mesenteroides has been used: • in the coupled spectrophotometric assay for PGI (F6P to G6P reaction) • to study the starch time course using Nicotiana tabacum leaves • to determine the glucose content in the root and stem samples of Quercus velutina Lam. saplings • to assay glucose using a starch sample of wild-type and transgenic Arabidopsis whole seedlings |
| Biochem/physiol Actions: | Glucose-6-phosphate dehydrogenase (G6PD) catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway. |
| Biochem/physiol Actions: | Glucose-6-phosphate dehydrogenase (G6PD) is expressed in all tissues and produces the reduced form of nicotinamide adenine dinucleotide phosphate (NADPH), which aids the cells to prevent oxidative stress. Insufficiency of G6PD is an X-linked, hereditary genetic defect. It is caused due to a common mutation in the G6PD gene. |
| Other Notes: | One unit will oxidize 1.0 μmole of |
| Packaging: | 200, 1000 units in glass bottle |
| Packaging: | 2000, 10000 units in poly bottle |
| Physical form: | Lyophilized powder containing Ficoll and Tris buffer salts |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥550 units/mg protein (biuret) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 1.1.1.49 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |