Glutaminase from Escherichia coli
SIGMA/G8880 - Grade V, lyophilized powder, ≥50 units/mg protein
Synonym: L-Glutamine amidohydrolase
CAS Number: 9001-47-2
MDL Number: MFCD00131193
Product Type: Chemical
Unit Definition One unit will deaminate 1.0 μmole of L-glutamine per min at pH 4.9 at 37 °C.
| biological source | Escherichia coli |
| cation traces | NH4+: <0.1 μg/unit |
| composition | Protein, ~30% biuret |
| foreign activity | NADH Oxidase ≤0.01% |
| form | lyophilized powder |
| mol wt | 110 kDa |
| Quality Level | 200  |
| solubility | water: 1 mg/mL (Clear w/Particle to Sl. Hazy w/Particles) |
| specific activity | ≥50 units/mg protein |
| storage temp. | −20°C |
| type | Grade V |
| Application: | Glutaminase from E.coli has been used: • for the determination of glutamine from the cerebral cortex tissue extract • for immobilization on aminopropylsilylated controlled pore glass (CPG) for determination of glutamine • in combination with acivicin in in vitro melanoma cells and MCF-7 and OAW-42 cells for cell proliferation and invasiveness assay. |
| Biochem/physiol Actions: | Glutaminase catalyzes the conversion of glutamine to glutamate. |
| Biochem/physiol Actions: | Glutaminase from Escherichia coli along with acivicin, a glutamine analog regulates proliferation and invasiveness of cancer cells. Bacterial glutaminase is implicated in increasing the life span of cancer patients by lowering the tumor burden. |
| General description: | Glutaminase is an amidohydrolase. It has the catalytic domain in the N-terminal and the C-terminal extension possesses STAS domain. |
| Other Notes: | One unit will deaminate 1.0 μmole of |
| Packaging: | 10 units in poly bottle |
| Packaging: | 50 units in glass bottle |
| Physical form: | Lyophilized powder containing potassium succinate and EDTA |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥50 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.5.1.2 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |