Anti-GRP78/BiP (GL-19) antibody produced in rabbit
SIGMA/G8918 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-78-kDa Glucose-Regulated Protein; Anti-Immunoglobulin Heavy Chain Binding Protein
MDL Number: MFCD01865530
Product Type: Chemical
Western Blotting JOURNAL CITATION: Biochemical evidence that regulation of Ero1β: activity in human cells does not involve the isoform-specific cysteine 262. By: Hansen, H. G., Søltoft, C. L., et al. in Biosci Rep, 2014. PubMed ID: 27919037 Image collected and cropped by CiteAb from the following publication, (http://bioscirep.org/cgi/doi/10.1042/BSR20130124), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: mTOR-Controlled Autophagy Requires Intracellular Ca(2+) Signaling. By: Decuypere, J. P., Kindt, D., et al. in PLoS One, 2013. PubMed ID: 23565295 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0061020), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunocytochemistry-immunoflourescence JOURNAL CITATION: Characterisation of a C1qtnf5 Ser163Arg knock-in mouse model of late-onset retinal macular degeneration. By: Shu, X., Luhmann, U. F., et al. in PLoS One, 2011. PubMed ID: 22110650 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0027433), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunoblot Whole cell extract of HeLa (lanes A, D) and NIH3T3 (lane B), and recombinant hamster GRP78 protein (Lane C) were separated on SDS-PAGE and blotted with Rabbit Anti-GRP78/BiP (GL-19) (Cat. No. G8918). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution 1:3,000. Lane D: Antibody dilution 1:3,000 + Inhibition with GRP78 immunizing peptide (20 μg/mL).
Enhanced Validation-By Independent Antibodies Immunofluorescence. 3T3 cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1:1,000 Anti-GRP78/BiP (GL-19) antibody produced in Rabbit (Cat. No. G8918) (A). The antibody developed using Goat Anti-Rabbit IgG, FITC conjugate antibody (Cat. No. F9887), and 1:1,000 Anti-GRP78/BiP (ET-21) antibody produced in Rabbit (Cat. No. G9043) (B). The antibody was developed using Goat Anti-Rabbit IgG, FITC conjugate antibody (Cat. No. F9887). Results Two Anti-GRP78/BiP antibodies, G8918 (A) and G9043 (B), target different regions of GRP78/BiP show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen 78 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | mouse, hamster, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: 1:1,000 using mouse fibroblasts NIH3T3 cells |
| microarray: suitable | |
| western blot: 1:3,000 using human epitheloid carcinoma HeLa whole cell extract | |
| UniProt accession no. | P11021  |
| Application: | Anti-GRP78/BiP (GL-19) antibody produced in rabbit has been used in: • western blotting • immunocytochemistry • immunohistochemistry |
| Biochem/physiol Actions: | GRP78 (78-kDa glucose-regulated protein) is essential for cell viability. GRP78/BiP is induced by stress-inducing agents or by conditions that adversely affect endoplasmic reticulum(ER) function, including oxidative stress, chemical toxicity, Ca2+ ionophores, inhibition of the ER Ca2+ -ATPase, inhibitors of glycosylation and hypoxia. GRP78/BiP is critical for maintenance of Ca2+ homeostasis and the prevention of apoptosis. GRP78/BiP levels are reduced in Alzheimer′s disease brains and the decreased expression of GRP78/BiP is found associated with mis-sense mutations in presenilin-1 (PS-1). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | GRP78/BiP is a Ca2+-binding molecular chaperone located in the endoplasmic reticulum (ER). It is a highly conserved protein. |
| Packaging: | 200 μL in glass bottle |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |