Anti-Glial Fibrillary Acidic Protein antibody produced in rabbit
SIGMA/G9269 - IgG fraction of antiserum, buffered aqueous solution
Synonym: GFAP Antibody Sigma - Anti-Glial Fibrillary Acidic Protein antibody produced in rabbit; Gfap Antibody Sigma; Anti-GFAP
MDL Number: MFCD00145904
Product Type: Chemical
Western Blotting JOURNAL CITATION: Obesity and diabetes cause cognitive dysfunction in the absence of accelerated β:-amyloid deposition in a novel murine model of mixed or vascular dementia. By: Niedowicz, D. M., Reeves, V. L., et al. in Acta Neuropathol Commun, 2014. PubMed ID: 24916066 Image collected and cropped by CiteAb from the following publication, (http://actaneurocomms.biomedcentral.com/articles/10.1186/2051-5960-2-64), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunocytochemistry JOURNAL CITATION: The P2X7 receptor supports both life and death in fibrogenic pancreatic stellate cells. By: Haanes, K. A., Schwab, A., et al. in PLoS One, 2013. PubMed ID: 23284663 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0051164), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistology
Specific staining of astrocytes with formalin-fixed, paraffin-embedded section of rat cerebellum using Rabbit Anti-GFAP (Cat. No. G9269). Antibody dilution 1:80.
Specific staining of astrocytes with formalin-fixed, paraffin-embedded section of rat cerebellum using Rabbit Anti-GFAP (Cat. No. G9269). Antibody dilution 1:80.
Immunofluorescence
Primary neuronal culture of rat amygdala. Nuclei stained blue with DAPI (Cat. No. D9542), neuron cluster (lower left) ensheathed by gail processes stained red with Rabbit Anti-GFAP (Cat. No. G9269) and Goat Anti-Rabbit IgG-TRITC (Cat. No. T6778) and positive neuronal fibers stained green with Rabbit Anti-Neurofilament 200 (Cat. No. N4142) and Goat Anti-Rabbit IgG-FITC (Cat. No. F9887).
From R. Gabr, West Virginia Univ., Dept of Pharmacology & Toxicology, Morgantown, WV.
Primary neuronal culture of rat amygdala. Nuclei stained blue with DAPI (Cat. No. D9542), neuron cluster (lower left) ensheathed by gail processes stained red with Rabbit Anti-GFAP (Cat. No. G9269) and Goat Anti-Rabbit IgG-TRITC (Cat. No. T6778) and positive neuronal fibers stained green with Rabbit Anti-Neurofilament 200 (Cat. No. N4142) and Goat Anti-Rabbit IgG-FITC (Cat. No. F9887).
From R. Gabr, West Virginia Univ., Dept of Pharmacology & Toxicology, Morgantown, WV.
Immunofluorescence
Glial cells cultured from rat amygdala. Nuclei stained blue with DAPI (Cat. No. D9542) and glial processes stained green with Rabbit Anti-GFAP (Cat. No. G9269) and Goat Anti-Rabbit IgG-FITC (Cat. No. F9887).
From R. Gabr, West Virginia University, Dept of Pharmocology and Toxicology, Morgantown, WV.
Glial cells cultured from rat amygdala. Nuclei stained blue with DAPI (Cat. No. D9542) and glial processes stained green with Rabbit Anti-GFAP (Cat. No. G9269) and Goat Anti-Rabbit IgG-FITC (Cat. No. F9887).
From R. Gabr, West Virginia University, Dept of Pharmocology and Toxicology, Morgantown, WV.
Immunoblotting
Rat brain whole cell estracts were separated on SDS-PAGE, blotted with Rabbit Anti-GFAP (Cat. No. G9269) and developed using Goat Anti-Rabbit IgG, Peroxidase (Cat. No. A0545) and AEC substrate. (Lane A) Antibody dilution 1:200; (Lane B) Negative control (only secondary antibody).
Rat brain whole cell estracts were separated on SDS-PAGE, blotted with Rabbit Anti-GFAP (Cat. No. G9269) and developed using Goat Anti-Rabbit IgG, Peroxidase (Cat. No. A0545) and AEC substrate. (Lane A) Antibody dilution 1:200; (Lane B) Negative control (only secondary antibody).
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:80 using brain sections |
| microarray: suitable | |
| western blot: 1:500 using brain extract | |
| UniProt accession no. | P14136  |
| Application: | Anti-Glial Fibrillary Acidic Protein antibody produced in rabbit is suitable for: • immunohistochemical staining at a working dilution of 1:400 using rat brain sections • immunohistochemical staining of human brain tumor tissue specimens to identify the cells expressing AQP4 (Aquaporin-4) • immunofluorescence using brain section from mice The antibody is suitable for immunohistochemistry at a working dilution of 1:80 using alcohol- and formalin-fixed, paraffin-embedded sections of human or animal brain tissue. It is also suitable for indirect immunoblotting at a working dilution of 1:500 using human brain extract blot and microarray. |
| Biochem/physiol Actions: | Glial fibrillary acidic protein may be used as a marker for distinguishing astrocytes from other glial cells during development of the central nervous system. GFAP is responsible for maintaining the structure of glial cells. It also offers mechanical strength and support to the surrounding neurons and blood brain barrier. Defects in this gene causes Alexander disease, indicated by Rosenthal fibers deposition. It is a rare disorder of astrocytes in the central nervous system. Increased GFAP expression indicates astrogliosis, a sign of neurodegeneration and neuroinflammation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The GFAP (glial fibrillary acidic protein) gene encodes an intermediate protein filament-III (50kDa). It is localized particularly to the astrocytes and non-myelinating Schwann cells of the central and peripheral nervous system, respectively. |
| Immunogen: | GFAP from human brain |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide |
| Specificity: | The product reacts specifically with GFAP and labels astrocytes, glial cells and glial cell derived tumors in immunohistochemical staining. The antibody reacts with glial specific antigen in alcohol or formalin-fixed, paraffin-embedded human or animal tissue sections. |
| Storage and Stability: | Store at 2-8 °C up to one month. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |