Guanidine thiocyanate
SIGMA/G9277 - Molecular Biology, ≥99%
Synonym: Guanidinium rhodanide; Guanidinium thiocyanate
CAS Number: 593-84-0
Empirical Formula (Hill Notation): CH5N3 · CHNS
Molecular Weight: 118.16
EC Number: 209-812-1
MDL Number: MFCD00013027
Linear Formula: NH2C(=NH)NH2 · HSCN
Product Type: Chemical
ATR-IR Spectra for Guanidinium rhodanide.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: G9277-100G
This image depicts SKU: G9277-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: G9277-250G
This image depicts SKU: G9277-250G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: G9277-500G
This image depicts SKU: G9277-500G
| application(s) | agriculture |
| assay | ≥99% |
| biological source | synthetic (organic) |
| cation traces | Ba: ≤5 ppm |
| Fe: ≤5 ppm | |
| heavy metals (as Pb): ≤5 ppm | |
| Na: ≤0.5% | |
| color | colorless to white, |
| density | 1.29 g/cm3 at 20 °C (68 °F) |
| foreign activity | DNase, RNase, none detected |
| form | powder or crystals |
| grade | Molecular Biology |
| InChI | 1S/CH5N3.CHNS/c2-1(3)4;2- |
| InChI key | ZJYYHGLJYGJLLN-UHFFFAOYSA |
| mp | 120-122 °C (lit.) |
| Quality Level | 200  |
| SMILES string | SC#N.NC(N)=N |
| solubility | water: 100 mg/mL, clear, colorless |
| storage condition | ( |
| technique(s) | RNA extraction: suitable |
| Application: | Chaotropic agent and strong denaturant; solubilizes cells. |
| Application: | Guanidine thiocyanate has been used: • for the lysis of colorectal adenocarcinoma cell line prior to RNA isolation • as a PCR inhibitor in triplex mitochondrial DNA (mtDNA) qPCR assay • for DNA extraction from cerebrospinal fluid (CSF) |
| Biochem/physiol Actions: | Guanidine thiocyanate is a denaturing agent and is used routinely in RNA isolation. It is used as a storage buffer for whole blood samples. Guanidine thiocyanate inactivates nucleases and is ideal for storing and freezing fecal samples for DNA studies. It is used in combination with phenol−chloroform in RNA extraction. |
| Features and Benefits: | • Highly versatile surfactant for your cell biology and biochemical research • Suitable for RNA extraction in sensitive molecular biology applications • Free from Dnase and Rnase • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications |
| General description: | Guanidine thiocyanate, or guanidinium rhodanide, is a versatile chemical compound widely used in molecular biology and biochemical research. Acting as a chaotropic agent, it disrupts cell and organelle membranes, facilitating cell lysis and organelle isolation. Notably, it serves as a potent protein denaturant, surpassing guanidine hydrochloride in unfolding proteins. This property is valuable for purifying nucleic acids from crude cell lysates, effectively separating nucleic acids from denatured proteins. In nucleic acid purification, guanidine thiocyanate is effective in inactivating DNases and RNases, preventing nucleic acid degradation and ensuring favorable yields. Its dual denaturing and enzyme-inhibiting properties make it crucial for DNA and RNA studies. Beyond nucleic acid isolation, guanidine thiocyanate is used for cell lysis and virus particle inactivation during RNA and DNA extractions. It not only disrupts membranes but also denatures RNase and DNase enzymes, protecting extracted nucleic acids from enzymatic degradation. The versatility of guanidine thiocyanate extends to molecular biology techniques like PCR, Northern blotting, and Southern blotting. It serves as a vital reagent in these methods, contributing to the successful analysis and amplification of nucleic acids. |
| Other Notes: | For additional information on our range of Biochemicals , please complete this form . |
| Packaging: | 100, 250, 500 g in poly bottle |
| Symbol |   GHS05,GHS07 |
| Signal word | Danger |
| Hazard statements | H302 + H312 + H332 - H314 - H412 |
| Precautionary statements | P260 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 |
| Hazard Codes | C,Xn |
| Risk Statements | 20/21/22-32-34-52/53 |
| Safety Statements | 13-26-36/37/39-45-61 |
| RIDADR | UN 1759 8 / PGIII |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Supplemental Hazard Statements | EUH032 |
| Purity | ≥99% |
| mp | 120-122 °C (lit.) |
| Density | 1.29 g/cm3 at 20 °C (68 °F) |
| UNSPSC | 12352107 |