Monoclonal Anti-Glutamate antibody produced in mouse
SIGMA/G9282 - clone GLU-4, ascites fluid
MDL Number: MFCD00162331
Product Type: Chemical
ELISA Monoclonal Anti-Glutamate antibody produced in Mouse, Clone: GLU-4 (Cat. No. G9282), followed by 1:1,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917), was serially diluted to detect Glutamate by indirect ELISA.
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | GLU-4, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | wide range |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: 1:10,000 using in situ prepared |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immuno-electron microscopy (1 paper) |
| Application: | Monoclonal Anti-Glutamate antibody produced in mouse is suitable for: • immunolabeling of glutamate in the lamina of the Drosophila visual system • immunocytochemistry at a working dilution of 1:20,000 to detect glutamate using heart and extrinsic nerves of stomatopod crustacean, Squilla oratoria • immunofluorescent staining as a primary antibody at a working dilution of 1:1000 using cryosections of brains and embryos from mice • use as a primary antibody to measure the glutamate release in synaptosome preparations It is also suitable for indirect ELISA at a working dilution of 1:10,000 using in situ prepared L-glutamate-glutaraldehyd |
| Biochem/physiol Actions: | Glutamate is an amino acid that acts as a excitatory neurotransmitter in the vertebrate brain and functions in all brain processes. It is released from one end of the neuron and it binds to receptors on the surface of adjacent neurons. Glutamate is released in a calcium-dependent manner in response to depolarizing stimuli. It serves as a substrate in certain metabolic pathways, such as, synthesis of glutamine and γ-aminobutyric acid (GABA) Excessive release of glutamate or aspartate have been observed in the pathogenesis of certain neurodegenerative diseases, such as, Huntington’s disease, Alzheimer’s disease. The same is also observed in selective neuronal degenerations involved in epilepsy, ischemia, and hypoglycemia. |
| Biochem/physiol Actions: | The antibody reacts specifically with |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |