Guanidine hydrochloride solution
SIGMA/G9284 - Colorless liquid, 7.8 - 8.3 M, pH- 4.5 - 5.5
Synonym: Aminoformamidine hydrochloride; Guanidinium chloride; Guanidium chloride
CAS Number: 50-01-1
Empirical Formula (Hill Notation): CH5N3 · HCl
Molecular Weight: 95.53
MDL Number: MFCD00013026; MFCD00013026
Linear Formula: CH5N3 · HCl
Product Type: Chemical
A representative image of the product packaging
ATR-IR Spectra for Aminoformamidine hydrochloride.
| density | 1.18 g/mL at 25 °C (lit.) |
| form | liquid |
| InChI | 1S/CH5N3.ClH/c2-1(3)4;/h( |
| InChI key | PJJJBBJSCAKJQF-UHFFFAOYSA |
| Quality Level | 200  |
| SMILES string | Cl.NC(N)=N |
| Application: | Guanidine hydrochloride solution has been used: • for protein denaturation • to homogenize hypothalamus, thalamus, prefrontal cortex, and brainstem samples for total β-amyloid (Aβ) extraction and Aβ42 quantification • as a chaotropic denaturant to treat human immunodeficiency virus-1 (HIV-1) samples to study its effects on HIV-1 sensitivity and to assay protein stability |
| Biochem/physiol Actions: | Guanidine‐HCl (Gnd-HCl), an efficient chaotropic agent, is broadly used in RNA isolation, globular protein denaturation, and protein refolding studies as a denaturant. It is an appropriate sample buffer for the quick and effective elution of affinity-purified protein complexes and the subsequent protein digestion step. GnHCl can be used to extract proteins in extracellular matrix (ECM)‐rich tissues including bone, cartilage, ligament, and tendon. Gnd-HCl is not used in proteomics experiments due to its interference with trypsin activity at low molar concentrations and tendency to precipitate when combined with sodium dodecyl sulfate (SDS). It is appropriate for performing rapid and single-step affinity-purification mass spectrometry (AP-MS) experiments. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H302 + H332 - H315 - H319 |
| Precautionary statements | P261 - P264 - P301 + P312 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 22-36/38 |
| Safety Statements | 26 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Density | 1.18 g/mL at 25 °C (lit.) |
| UNSPSC | 12352107 |