Anti-GAL4, Activation domain antibody produced in rabbit
SIGMA/G9293 - ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Galactose 4
MDL Number: MFCD01633372
Product Type: Chemical
Immunoblotting
An extract of S. cerevisiae, strain Y187, expressing GAL4-AD protein (Lanes 1, 2) was separated on SDS-PAGE, blotted with Rabbit Anti-GAL4 (Cat. No. G9293) and developed using Goat Anti-Rabbit IgG-Peroxidase and chemiluminescent substrate. (Lane 1) Antibody concentration 1 μg/mL; (Lane 2) Antibody concentration 1 μg/mL + 10 μg/mL GAL4AD immunizing peptide.
An extract of S. cerevisiae, strain Y187, expressing GAL4-AD protein (Lanes 1, 2) was separated on SDS-PAGE, blotted with Rabbit Anti-GAL4 (Cat. No. G9293) and developed using Goat Anti-Rabbit IgG-Peroxidase and chemiluminescent substrate. (Lane 1) Antibody concentration 1 μg/mL; (Lane 2) Antibody concentration 1 μg/mL + 10 μg/mL GAL4AD immunizing peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 0.5 μg/mL using GAL4-AD fusion protein in Saccharomyces cerevisiae extract |
| Application: | Anti-GAL4, Activation domain antibody produced in rabbit has been used in western blotting. |
| Biochem/physiol Actions: | GAL4 induces the genes that are responsible for regulation of galactose metabolism in saccharomyces cerevisiae. GAL4 protein is widely used for studying transcriptional activation in eukaryotes. The yeast two hybrid system is widely used to study to discover protein− protein interactions (PPIs) and protein−DNA interactions. |
| Disclaimer: | Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | GAL4 protein is an 881 amino acid transcription factor containing an independent N-terminal DNA binding (147 amino acids) and C-terminal activator domains. It is a regulatory gene that belongs to GAL gene family. It is a component of yeast two hybrid system, where GAL 4 DNA-BD is fused to protein X (bait) and GAL4 activation domain is fused to protein Y (prey). |
| General description: | Useful in the study of protein:protein interaction studies. GAL4 DNA activation domain fusion proteins. |
| Immunogen: | Synthetic peptide corresponding to amino acids of Saccharomyces cerevisiae GAL4 protein. |
| Physical form: | The product is provided as a solution of affinity isolated antibody in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide as a preservative. |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in “frost-free” freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |