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Monoclonal Anti-GAPDH−Peroxidase antibody produced in mouse

SIGMA/G9295 - clone GAPDH-71.1, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-G3PD; Anti-G3PDH; Anti-Glyceraldehyde-3-phosphate Dehydrogenase

MDL Number: MFCD09265360
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-G9295-200UL 200 µL
 $775.00
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Western Blotting JOURNAL CITATION: Modeling tandem AAG8-MEK inhibition in melanoma cells. By: Sun, B., Kawahara, M., et al. in Cancer Med, 2014. PubMed ID: 24634165 Image collected and cropped by CiteAb from the following publication, (http://doi.wiley.com/10.1002/cam4.233), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Modeling tandem AAG8-MEK inhibition in melanoma cells. By: Sun, B., Kawahara, M., et al. in Cancer Med, 2014. PubMed ID: 24634165 Image collected and cropped by CiteAb from the following publication, (http://doi.wiley.com/10.1002/cam4.233), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Modeling tandem AAG8-MEK inhibition in melanoma cells. By: Sun, B., Kawahara, M., et al. in Cancer Med, 2014. PubMed ID: 24634165 Image collected and cropped by CiteAb from the following publication, (http://doi.wiley.com/10.1002/cam4.233), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunoblotting Total cell extract of A431 cells was separated on SDS-PAGE and blotted with Monoclonal Anti-GAPDH, Peroxidase conjugate (Cat. No. G9295). The antibody was developed with chemiluminescent substrate. Lanes: 1. 1:20,000 antibody 2. 1:40,000 antibody 3. 1:60,000 antibody 4. 1:80,000 antibody

 

antibody form purified immunoglobulin
antibody product type primary antibodies
application(s) research pathology
biological source mouse
clone GAPDH-71.1, monoclonal
conjugate peroxidase conjugate
form buffered aqueous solution
isotype IgM
mol wt antigen ~37 kDa
Quality Level 200 
shipped in dry ice
should not react with prokaryotes
species reactivity rabbit, canine, rat, hamster, monkey, mouse, turkey, bovine, mink, human, chicken
storage temp. −20°C
target post-translational modification unmodified
technique(s) western blot: 1:35,000-1:70,000 using A431 total cell extract
UniProt accession no. P04406 
Application: Monoclonal Anti-GAPDH-Peroxidase antibody produced in mouse has been used in
• immunoblotting
• enzyme linked immunosorbent assay (ELISA)
• immunocytochemistry
Biochem/physiol Actions: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a tetramer containing identical chains. It catalyzes the reversible oxidative phosphorylation of glyceraldehyde-phosphate, which is a critical energy-yielding step in carbohydrate metabolism. It binds to several proteins including actin, tubulin, amyloid precursor, polyglutamine peptides, DRPLA (dentatorubral-pallidoluysian atrophy), and huntingtin. Protein kinase Cι/λbinds and phosphorylates GAPDH. Phosphorylated GAPDH associates with cytoskeletal elements and controls microtubule dynamics in the early secretory pathway. Poly(ADP-ribose) polymerase-1 (PARP1) interacts with GAPDH and thereby mediates brain damage in the presence of oxidative/nitrosative stress. GAPDH forms a part of OCA-S, the multicomponent OCT1 (octamer-motif-binding factor) coactivator complex, which is involved in the S phase-dependent histone H2B transcription. This association is responsible for linking H2B transcriptional machinery to cell cycle regulation and to the cellular metabolic state. GAPDH is also a component of the functional GAIT (interferon-γ-activated inhibitor of translation) mRNP (messenger ribonucleoprotein). GAPDH expression is dysregulated during melanoma progression.
Biochem/physiol Actions: The antibody does not crossreact with non-vertebrate and prokaryote GAPDH.
Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description: Monoclonal Anti-GAPDH (mouse IgM isotype) is derived from the hybridoma GAPDH-71.1 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with rabbit GAPDH.
General description: The gene glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is mapped to human chromosome 12p13. The protein localizes in the cytoplasm but can be translocated to the nucleus depending on cellular conditions.
Immunogen: rabbit GAPDH.
Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT.
Storage Temp. −20°C
UNSPSC 12352203

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