Glycerol 3-phosphate Oxidase from Pediococcus sp.

SIGMA/G9637 - lyophilized powder, 40-80 units/mg solid, pH 8.1

Synonym: sn-Glycerol 3-phosphate:oxygen 2-oxidoreductase; L-Glycerol 3-phosphate Oxidase

CAS Number: 9046-28-0
EC Number: 232-932-0
MDL Number: MFCD00131209
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-G9637-100UN	100 units	$149.00 1/EA	Add To Favorites

Figure 1. Stability (powder form); kept under dry conditions

Figure 2. pH Activity; 30 °C, 10-min. reaction in 50 mM buffer solution; pH 6.0–7.0, MES; pH 7.0–9.5, Tris-HCl; pH 9.0–10.5, K₂CO₃-NaHCO₃

Figure 3. Temperature activity; in 50 mM Tris-HCl buffer, pH 8.1

Figure 4. pH Stability; 25 °C, 20-hr treatment with 0.1 M buffer solution: pH 5.0–7.0, dimethyglutaric acid-NaOH; pH 7.0–8.8, K-phosphate; pH 8.8–10.0, K₂CO₃-NaHCO₃.

Figure 5. Thermal stability; 15-min. treatment with 0.1 M dimethyglutaric acide-NaOH buffer, pH 7.0.

Properties
Descriptions
Safety Info.
Basic Data

biological source	bacterial (Pediococcus spp.)
form	lyophilized powder
mol wt	~76 kDa (gel filtration)
Quality Level	200
specific activity	40-80 units/mg solid, pH 8.1
storage temp.	2-8°C

Application:	Glycerol 3-phosphate Oxidase from Pediococcus sp. has been used in the kinetic assay to study gluconeogenesis in liver and in immobilization studies on pencil graphite (PG) electrode.
Application:	This enzyme is useful for enzymatic determination of triglyceride when coupled with lipoprotein lipase (LPL-311, LPL-314) and glycerokinase (GYK-301, GYK-311) in clinical analysis.
General description:	Glycerol 3-phosphate Oxidase is a FAD-dependent α-glycerophosphate oxidase and catalyzes the formation of dihydroxyacetonephosphate. Glycerol 3-phosphate oxidase comprises of a flavin adenine dinucleotide (FAD) and a substrate binding domain and corresponds to molecular weight of 42 kDa. It exists as a monomer and the active site comprises of isoalloxazine ring and histidine 53, arginine 316 and lysine 345 residues crucial for ligand binding.
General description:	Isoelectric point : 4.1 -/+0.1 Michaelis constants : 3.2x10 ^-3M (L-α-Glycerophosphate), 6.8 x 10 ^-3M (D, L-form) Inhibitors : Ionic detergents (SDS, LBS, etc.), Hg⁺⁺, Ag⁺ Optimum pH : 8.0 - 8.5Optimum temperature : 35 - 40o^C pH Stability : pH 6.5 - 8.5 (25o^C, 20hr) Thermal stability : below 40oC(pH 7.0, 15min) Substrate specificity : The enzyme has the highest specificity for L-form of a-γlycerophosphate
Other Notes:	One unit will oxidize 1.0 μmole of L-glycerol 3-phosphate to dihydroxyacetone phosphate with the formation of H₂O₂ per min at 37°C, at the appropriate pH.
Packaging:	100 units in glass bottle
Physical form:	Lyophilized powder containing stabilizers

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

activity	specific activity: 40-80 units/mg solid, pH 8.1
Storage Temp.	2-8°C
Enzyme Commission (EC) Number	1.1.3.21 ( BRENDA \| IUBMB )
UNSPSC	12352204

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