Glucose (HK) Assay Kit
SIGMA/GAHK20 - sufficient for 20 assays
Synonym: Glucose Quantification Kit
Product Type: Chemical
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| analyte chemical class(es) | sugars (glucose) |
| application(s) | food and beverages general analytical |
| Quality Level | 100  |
| storage temp. | 2-8°C |
| technique(s) | photometry: suitable |
| usage | sufficient for 20 assays |
| Application: | This kit is for the quantitative, enzymatic determination of glucose in food and other material. Glucose is phosphorylated by adenosine triphosphate (ATP) in the reaction catalyzed by hexokinase. Glucose-6-phosphate (G6P) is then oxidized to 6-phospho-gluconate in the presence of oxidized nicotinamide adenine dinucleotide (NAD) in a reaction catalyzed by glucose-6-phosphate dehydrogenase (G6PDH). During this oxidation, an equimolar amount of NAD is reduced to NADH. The consequent increase in absorbance at 340 nm is directly proportional to glucose concentration. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Storage Temp. | 2-8°C |
| UNSPSC | 12164500 |
| Components | glucose (HK) assay reagent 20 mL; glucose standard .5 mL |