Grace Bio-Labs ONCYTE® SuperNOVA™ nitrocellulose film slides
SIGMA/GBL705024 - 24-5 mm x 6 mm pads per slide, plain glass, barcoded
Synonym: Grace Biolabs 705024; film slides; nitrocellulose slides; porous nitrocellulose film slides; protein microarrays
Product Type: Equipment/Book
Dimensions are in millimeters and are for reference only.
| binding type | high binding surface |
| external size | 25 mm × 75 mm |
| manufacturer/tradename | Grace Bio-Labs 705024 |
| material | nitrocellulose (on glass) |
| packaging | pkg of 20 ea |
| Application: | Grace Bio-Labs ONCYTE® SuperNOVA™ nitrocellulose film slides might be suitable for: • antigen or antibody capture • cell or tissue lysate binding • glycoprotein and peptide binding assay |
| Features and Benefits: | • Packed in boxes with minimal off-gassing • Require no pre-processing prior to array spotting • Ready for printing straight from the box • Require no activation steps to immobilize proteins • Can be analyzed using various laser scanners or colorimetric or fluorescence detectors (CCD) imaging systems |
| General description: | Fluorescence detection is a preferred method for many quantitative protein microarray assays in research and diagnostic applications due to its excellent sensitivity and potential for multiplexing. Use of multiplexed fluorescence-based assays on porous nitrocellulose though has been hampered by high backgrounds at shorter excitation wave lengths (such as the 532nm fluorescence channel). These ONCYTE™ SuperNOVA™ microporous nitrocellulose film slides increase protein microarray assay sensitivity and dynamic range using fluorescent end points at multiple wave lengths. Use of SuperNOVA™ slides can lower the limit of detection with fluorescent end points by 3 – 4 orders of magnitude over competing nitrocellulose film slides. |
| General description: | Porous nitrocellulose (PNC) film slides are the major high binding protein microarray substrate technology. Grace Bio-Labs ONCYTE® nitrocellulose film slides offer a significantly higher surface area for protein binding. These slides are compatible with many detection methods. Fluorescent, chemiluminescent, isotopic, and chromogenic detection methods that are commonly employed for Western and Northern blots are compatible with porous nitrocellulose films. ONCYTE® film slides need not require any activation step to immobilize proteins and are printable straight from the box. Pre-wetting ONCYTE® film slides should be avoided as printing onto a wet slide will result in the sample spreading, resulting in larger, more diffuse spots. |
| Legal Information: | NOVA is a trademark of Grace Bio-Labs, Inc. |
| Legal Information: | Oncyte is a registered trademark of Grace Bio-Labs, Inc. |
| Legal Information: | SuperNOVA is a trademark of Grace Bio-Labs, Inc. |
| UNSPSC | 41122600 |