His GraviTrap™
SIGMA/GE11-0033-99 - Cytiva 11-0033-99
Synonym: His GraviTrap Column
Product Type: Chemical
| average diameter | 90 μm |
| bed volume | 1 mL |
| capacity | ~40 mg binding capacity (histidine-tagged protein/column)(Protein binding capacity is protein-to-protein dependent.) |
| ~40 mg binding capacity(histidine-tagged protein) | |
| cleaning | 2-14(Ni2+-stripped medium) |
| column I.D. | 15 mm |
| manufacturer/tradename | Cytiva 11-0033-99 |
| matrix | 6% cross-linked agarose |
| packaging | pkg of 10 ea |
| particle size | 45-165 μm |
| suitability | suitable for bioprocess medium |
| working range | 3-12(Ni2+-stripped medium.) |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Features and Benefits: | • For fast and simple purification of histidine-tagged* proteins. • Convenient, prepacked gravity-flow column. • Allows direct purification of both clarified and unclarified cell lysates. • High binding capacity; approximately 40 mg of pure protein per column. • Compatible with a wide range of reducing agents, detergents, denaturants and other additives. |
| General description: | His GraviTrap™ is a prepacked, single-use column for purification of histidine-tagged proteins by immobilized metal affinity chromatography (IMAC). Column gives fast and simple gravity-flow purifications without any need for a purification. The ready to use column is prepacked with Ni Sepharose™ 6 Fast Flow, which has a high protein binding capacity, low nickel-ion leakage, and is compatible with denaturing and reducing agents, as well a wide range of additives. His GraviTrap™ columns are delivered in a package that can be converted into a column stand to simplify purification. LabMate PD-10 Buffer Reservoir can be connected to the columns for convenient handling of sample volumes above 10 mL. |
| Legal Information: | GraviTrap is a trademark of Cytiva |
| Legal Information: | Sepharose is a trademark of Cytiva |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Preparation Note: | Store at 4 to 30 °C (20% Ethanol) |
| Symbol |  GHS09 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | NONH for all modes of transport |
| UNSPSC | 12161501 |