Sephadex® G-50
SIGMA/GE17-0043-02 - Medium, Cytiva, pack of 500 g
Synonym: SEC resin; buffer exchange resin; chromatography resin; cross-linked dextran; cytiva 17-0043-02; desalting matrix; filtration gel; size-exclusion resin
CAS Number: 9004-54-0
MDL Number: MFCD23701222.obs1210407
Product Type: Chemical
| cleaning in place | 2-13 |
| crosslinking | cross-linked |
| feature | autoclavable In wet form (pH 7.0) at 120°C for 30 min |
| form | resin |
| InChI | 1S/C18H32O16/c19-1-5(21)9 |
| InChI key | FZWBNHMXJMCXLU-UHFFFAOYSA |
| manufacturer/tradename | Cytiva |
| matrix | cross-linked dextran |
| matrix active group | dextran |
| mol wt | Mp 0.5-10 kDa (dextrans fractionation range) |
| protein Mr 3-20 kDa (fractionation range) | |
| protein Mr 30 kDa (exclusion limit) | |
| packaging | pack of 500 g |
| particle size | 103-320 μm (wet) |
| 50-150 μm (dry) | |
| separation technique | size exclusion (SEC) |
| shipped in | ambient |
| SMILES string | O1C(C(C(C(C1CO)O)O)O)OCC2 |
| storage temp. | 2-30°C |
| swelling | 1 g swells to 14-15 mL |
| technique(s) | liquid chromatography (LC): suitable |
| working range | 2-12 |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Features and Benefits: | • Quickly desalts, removes contaminants and transfers to a new buffer in a single step. • Suitable for purification of DNA from small molecules by gel filtration • Larger bead size for large scale group separations where high flow rates and low operating pressures are required. • Classic gel filtration medium. |
| General description: | Sephadex® G-50 Medium is well established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight. The larger bead size of G-50 Medium is suited for large scale separations. Sephadex® is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex® differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex® G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex® G-50 is available in 4 different particle sizes (Course, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Cource and Medium are are preferred for large scale group separations where high flow rates and low operating pressures are required. |
| Legal Information: | Sephadex is a registered trademark of Cytiva |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Preparation Note: | Store at 4 to 30 °C () |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | 2-30°C |
| UNSPSC | 41115722 |