Ficoll® PM 400
SIGMA/GE17-0300-50 - solid, spray-dried, solubility:, soluble, mol wt 300-500 kDa, non-sterile (endotoxins < 0.12 EU/mL), pack of 500 g
Synonym: 17-0300-50; Ficoll Density Gradient; cell culture media; cell purification media; cell storage media; gradient density media; lymphocyte separation medium; mononuclear cell isolation
Product Type: Chemical
| color | White |
| density | ≤1.2 g/mL |
| form | solid |
| spray-dried | |
| manufacturer/tradename | Cytiva 17-0300-50 |
| mol wt | 300-500 kDa |
| packaging | pack of 500 g |
| shelf life | 5 yr |
| solubility | soluble |
| sterility | non-sterile (endotoxins < 0.12 EU/mL) |
| storage temp. | 2-30°C |
| technique(s) | : suitable using Used to form density gradients for centrifugation seperation applications |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Application: | Used in the formation of density gradients for the separation and isolation of eukaryotic cells, organelles, and bacterial cells, as a stabilizing agent and as a separation medium for the isolation of lymphocytes. It also has applications in defined culture media, nucleic acid hybridization, electrophoresis, and immunological studies. Used for gradient centrifugation in all types of centrifuge rotors and for separation at unit gravity. |
| Features and Benefits: | • For producing density gradients for separation of cells and subcellular components by centrifugation or by sedimentation at unit gravity. • Neutral, highly branched, hydrophilic polymer of sucrose which dissolves readily in aqueous solution. • Concentrations of up to 50% (w/v) covering densities of up to 1.2 g/mL can be achieved. • Better osmotic properties than sucrose. • Preserves functional and morphological integrity. • Useful for separating cells that are sensitive to centrifugation and for separating cells of similar density but different size (under conditions of sedimentation at unit gravity). • Does not penetrate biological membranes. • Serves as the raw material for preparation of Ficoll-Paque gradients. • Used in other applications such as electrophoresis, hybridization, cryopreservation, and as a hapten carrier. |
| General description: | High molecular weight sucrose-polymers formed by copolymerization of sucrose with epichlorohydrin. The molecules are highly branched and the high content of hydroxyl groups leads to very good solubility in aqueous media. Ficoll® PM400 is a synthetic neutral, highly-branched hydrophilic polymer of sucrose with an average molecular weight of 400 000. It has long been used to form density gradients for separating and isolating eukaryotic cells, organelles and bacterial cells, as a stabilizing agent, and as a preparation medium for isolating mononuclear cells. Applications can also be found in defined culture media, nucleic acid hybridization, electrophoresis, and immunological studies. |
| General description: | It is delivered as a spray-dried powder and is therefore readily soluble in aqueous media when it is added slowly with concomitant stirring. Any lumps formed during this procedure will dissolve fairly quickly. Concentrations up to 50% (w/v) can easily be attained |
| Legal Information: | Ficoll is a registered trademark of Cytiva |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Preparation Note: | Store at 4 to 30 °C () |
| RIDADR | NONH for all modes of transport |
| Density | ≤1.2 g/mL |
| Storage Temp. | 2-30°C |
| UNSPSC | 41105330 |