HiTrap® Protein G High Performance
SIGMA/GE17-0405-01 - Cytiva 17-0405-01, pack of 1 × 5 mL
Synonym: Protein G affinity column; Protein purification column
Product Type: Chemical
HiTrap Protein G HP
| avg. part. size | 34 μm |
| bed volume | 5 mL |
| capacity | 25 mg binding capacity (human IgG / mL resin) |
| cleaning | 2-9 |
| crosslinking | cross-linked |
| form | prepacked column |
| I.D. × L | 16 mm × 25 mm |
| manufacturer/tradename | Cytiva 17-0405-01 |
| matrix | cross-linked agarose, spherical |
| matrix active group | Protein G |
| packaging | pack of 1 × 5 mL |
| parameter | 20 mL/min max. flow rate |
| 5 bar max. pressure | |
| 5 mL/min flow rate | |
| separation technique | affinity |
| storage condition | (20% Ethanol) |
| storage temp. | 2-8°C |
| technique(s) | liquid chromatography (LC): suitable |
| working range | 3-9 |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Features and Benefits: | • High binding capacity of 25 mg IgG/mL medium and fast binding kinetics. • 34 μm bead size for high performance, high-resolution purifications. • Binds broad range of different species and subclasses of IgG. • Simple and proven method giving reproducible results. • Designed for use with syringe, peristalic pump, or chromatography system. |
| General description: | HiTrap® Protein G HP columns are prepacked with Protein G Sepharose™ High Performance for fast, easy separations of IgG from different species and subclasses. |
| General description: | pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH. |
| Legal Information: | HiTrap is a registered trademark of Cytiva |
| Legal Information: | Sepharose is a trademark of Cytiva |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Symbol |  GHS02 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | UN1170 - class 3 - PG 3 - Ethanol, solution |
| Storage Temp. | 2-8°C |
| UNSPSC | 23151816 |