HiTrap® Heparin High Performance
SIGMA/GE17-0407-01 - Cytiva 17-0407-01, pack of 1 × 5 mL
Synonym: Heparin Affinity Column
Product Type: Chemical
HiTrap Heparin HP
| avg. part. size | 34 μm |
| bed volume | 5 mL |
| capacity | 3 mg binding capacity (antithrombin III / mL medium) |
| cleaning | 5-10 |
| crosslinking | cross-linked |
| I.D. × L | 16 mm × 25 mm |
| manufacturer/tradename | Cytiva 17-0407-01 |
| matrix | highly cross-linked 6% agarose |
| matrix active group | heparin |
| packaging | pack of 1 × 5 mL |
| parameter | 20 mL/min max. flow rate |
| 5 bar max. pressure | |
| 5 mL/min flow rate | |
| separation technique | affinity |
| shipped in | ambient |
| storage condition | (20% ethanol) |
| storage temp. | 2-30°C |
| technique(s) | liquid chromatography (LC): suitable |
| working range | 5-10 |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Features and Benefits: | • Heparin binds to a wide range of biomolecules via both affinity and ion exchange mechanisms. • Immobilized heparin is used for purification of antithrombin III, coagulation factors, and other plasma proteins. • Simple operation with a syringe, a pump or a chromatography system such as AKTA design • Stable over pH 5 to 10 and tolerates all commonly used aqueous buffers. |
| General description: | HiTrap® Heparin HP columns are prepacked with Heparin Sepharose™ High Performance for high-resolution purification of proteins with affnity for heparin such as DNA-binding proteins, coagulation factors, lipoproteins, and protein synthesis factors. Immobilized heparin has two main modes of interaction with proteins. It can operate as an affinity ligand with affinity for a wide range of biomolecules, including coagulation factors and other plasma proteins, lipoproteins, protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors. Heparin also functions as a high capacity cation exchanger, due to its anionic sulfate groups. Gradient elution with salt is most commonly used in both cases. Heparin is coupled to the Sepharose™ High Performance base matrix via the N-hydroxysuccinamide coupling method, to give high capacity, performance, and low leakage levels. HiTrap® Heparin HP is delivered in 0.2 M NaCl with 20% ethanol as bacteriostatic agent. Store HiTrap® Heparin HP columns equilibrated with 25 mL of 20% ethanol or a buffer containing a bacteriostatic agent. Equilibration is easily performed with a syringe and the supplied Luer adapter. Seal the column with the supplied stopper and domed nut. The recommended storage temperature is 4° C to 30° C. |
| Legal Information: | HiTrap is a registered trademark of Cytiva |
| Legal Information: | Sepharose is a trademark of Cytiva |
| Other Notes: | The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used. |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Preparation Note: | Store at 4 to 30 °C (20% Ethanol) |
| Symbol |  GHS02 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | UN1170 - class 3 - PG 3 - Ethanol, solution |
| Storage Temp. | 2-30°C |
| UNSPSC | 23151816 |