HiTrap® Chelating High Performance
SIGMA/GE17-0409-03 - Cytiva 17-0409-03, pack of 5 × 5 mL
Synonym: HiTrap Chelating Column
Product Type: Chemical
HiTrap Chelating HP
| avg. part. size | 34 μm |
| bed size | 16 mm × 25 mm |
| bed volume | 5 mL |
| cleaning | 2-14 |
| column I.D. | 16 mm |
| ligand | iminodiacetic acid |
| manufacturer/tradename | Cytiva 17-0409-03 |
| packaging | pack of 5 × 5 mL |
| parameter | <20 mL/min flow rate |
| 42 psi | |
| working range | 3-13 |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Features and Benefits: | • For optimizing purification of histidine-tagged proteins using different kinds of metal ions. • Conveniently charge with your metal of choice. • Iminidoacetic acid coupled to Sepharose™ High Performance. • Purifying proteins with exposed histidine groups via metal ion complex formation. • Simple operation with a syringe, pump or chromatographic system such as AKTA design or FPLC™ System. • Packed with Chelating Sepharose™ High Performance • Simple operation with a syringe, pump or chromatographic system such as AKTA design or FPLC™ System |
| General description: | HiTrap® Chelating HP is prepacked with Chelating Sepharose™ High Performance. The medium is charged with the metalion of your choice for Immobilized Metal ion Affinity Chromatography (IMAC) and subsequent purification of polyhistidine tagged proteins. HiTrap® Chelating HP is a prepacked ready to use, column for preparative affinity chromatography. The special design of the column, together with the matrix, provide fast, simple and easy separations in a convenient format. The columns can be operated with a syringe, peristaltic pump or liquid chromatography system such as AKTAdesign or FPLC™ Systems. |
| Legal Information: | FPLC is a trademark of Cytiva |
| Legal Information: | HiTrap is a registered trademark of Cytiva |
| Legal Information: | Sepharose is a trademark of Cytiva |
| Other Notes: | The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used. |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Preparation Note: | Store at 4 to 30 °C (20% Ethanol) |
| Symbol |  GHS02 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | UN1170 - class 3 - PG 3 - Ethanol, solution |
| UNSPSC | 23151816 |