Con-A Sepharose™ 4B
SIGMA/GE17-0440-03 - Cytiva 17-0440-03, pack of 5 mL
Synonym: chromatography resin; concanavalin A resin; gel; glycolipids affinity resin; glycoprotein affinity resin; matrix
Product Type: Chemical
Cytiva-11-0026-88
| average diameter | 90 μm |
| cleaning in place | 4-9 |
| ligand | concanavalin A (con A) |
| manufacturer/tradename | Cytiva 17-0440-03 |
| matrix | 4% agarose |
| packaging | pack of 5 mL |
| particle size | 45-165 μm |
| storage condition | (0.1 M acetate buffer pH 6, 1 M NaCl, 1 mM CaCl2, 1 mM MnCl2 and 1 mM MgCl, 20% Ethanol) |
| storage temp. | 2-8°C |
| working range | 4-6 |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Application: | Con A Sepharose™ is Concanavalin A coupled to Sepharose™ 4B by the cyanogen bromide method. Concanavalin A (Con A) is a tetrameric metalloProtein isolated from Canavalia ensiformis (jack bean). Con A binds molcules containing α-D-mannopyranosyl, α-D-glucopyranosyl and sterically related residues. The binding sugar requires the presence of C-3, C-4 and C-5 hydroxyl groups for reaction with Con A. Con A coupled to Sepharose™ is routinely used for separation and purification of glycoProteins, polysaccharides and glycolipids |
| Features and Benefits: | • Concanavalin A (Con A) binds molecules that contain α-D-mannose, α-D-glucose, and sterically related residues with available C-3, C-4, or C-5 hydroxyl groups • Group-specific adsorbent for molecules containing sugars. • Highly suitable for isolation of cell surface glycoProteins from detergent-solubilized membranes • Concanavalin A (Con A) binds molecules that contain α-D-mannose, α-D-glucose and sterically related residues with available C-3, C-4, or C-5 hydroxyl groups • Highly suitable for isolation of cell surface glycoProteins from detergent-solubilized membranes • Con A coupled to Sepharose™ 4B via cyanogen bromide activation |
| General description: | Con A Sepharose™ 4B with immobilized Concanavalin A is an affinity chromatography media for capture of glycosylated biomolecules including glycoproteins and glycolipids. Stronger affinity for α-D-glucose than α-D-mannose. |
| Legal Information: | Sepharose is a trademark of Cytiva |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Symbol |  GHS02 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | 2-8°C |
| UNSPSC | 41106500 |