Gammabind Plus Sepharose™
SIGMA/GE17-0886-01 - Cytiva 17-0886-01, pack of 5 mL
Synonym: Affinity chromatography resin; Protein A Sepharose
Product Type: Chemical
Cytiva-11-0026-88
| average diameter | 90 μm |
| cleaning in place | 2-9 |
| ligand | recombinant GammaBind G type 3 lacking albumin-binding region |
| manufacturer/tradename | Cytiva 17-0886-01 |
| matrix | 6% cross-linked agarose |
| packaging | pack of 5 mL |
| shipped in | wet ice |
| storage condition | (20% Ehtanol, PBS pH 7) |
| storage temp. | 2-8°C |
| working range | 3-9 |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Features and Benefits: | • Recombinant Protein G type 3 coupled to Sepharose™ CL-6B for enhanced binding to rat and mouse IgG subclasses at laboratory scale. • Binds to all IgG subclasses from human, mouse, and rat; binds total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. • Yields up to 18 mg/mL mouse IgG. |
| General description: | GammaBind Plus Sepharose™ is used for lab-scale affinity purification of IgG from a large variety of species and has better binding to rat and mouse IgG subclasses compared to GammaBind Sepharose. GammaBind Plus Sepharose™ is GammaBind G, Type 3, covalently immobilized to Sepharose™ CL-6B by malimide linkage. GammaBind G, Type 3, a recombinant form of streptococcal protein G, binds to the Fc region of IgG from a variety of mammalian species. GammaBind Plus Sepharose™ may be used to analyze and purify classes, subclasses and fragments of immunoglobulins from any biological fluid or cell culture medium. Since only the Fc region is involved in binding, the Fab region is still available for binding antigen. |
| General description: | pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH. |
| Legal Information: | Sepharose is a trademark of Cytiva |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Symbol |  GHS02 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | 2-8°C |
| UNSPSC | 41106500 |