NHS Act Sepharose™ 4 Fast Flow
SIGMA/GE17-0906-01 - Cytiva 17-0906-01, pack of 25 mL
Synonym: NHS-Activated Sepharose FF, pre-activated agarose matrix
Product Type: Chemical
| average diameter | 90 μm |
| cleaning in place | 2-13(ligand dependent) |
| crosslinking | cross-linked |
| form | resin |
| manufacturer/tradename | Cytiva 17-0906-01 |
| matrix | 4% cross-linked agarose |
| matrix active group | |
| operating pH | 2-9 |
| packaging | pack of 25 mL |
| parameter | 150-250 cm/hr flow rate (at 0.1 MPa) |
| particle size | 45-165 μm |
| separation technique | affinity |
| storage condition | (100% Isopropanol) |
| storage temp. | 2-8°C |
| technique(s) | liquid chromatography (LC): suitable |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Features and Benefits: | • NHS pre-activated medium for coupling of small amino-containing proteins and peptides in process-scale applications • High level of pre-activation gives a high degree of substitution of the selected ligand • NHS coupling method results in chemically stable ligand attachment • BioProcess medium supported for industrial applications |
| General description: | NHS-activated Sepharose™ 4 Fast Flow is a pre-activated agarose matrix that increases the choice of coupling chemistries available. Suitable for coupling amino-containing smaller proteins and peptides. NHS-activated Sepharose™ 4 Fast Flow is composed of cross-linked 4% agarose beads, NHS activated for coupling amino-containing proteins and peptides in process-scale applications. NHS (N-hydroxysuccinimide) coupling forms a chemically stable amide bond with ligands containing primary amino groups. This resin provides a spacer arm to the coupled ligand and is therefore particularly suitable for immobilising smaller protein and peptide ligands. NHS-activated Sepharose™ 4 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess media range, NHS-activated Sepharose™ 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support. |
| Legal Information: | Sepharose is a trademark of Cytiva |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Symbol |  GHS07 |
| Signal word | Danger |
| Hazard statements | H225 - H319 - H336 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P501 |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | 2-8°C |
| UNSPSC | 41106500 |