HiTrap® IMAC High Performance
SIGMA/GE17-0920-05 - Cytiva 17-0920-05, pack of 5 × 5 mL
Synonym: Metal affinity chromatography
Product Type: Chemical
HiTrap IMAC HP, 5 x 5 mL
| avg. part. size | 34 μm |
| bed size | 16 mm × 25 mm |
| bed volume | 5 mL |
| capacity | ≥40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.) |
| cleaning | 2-14(Medium uncharged with metal ions.) |
| column I.D. | 16 mm |
| manufacturer/tradename | Cytiva 17-0920-05 |
| matrix | highly cross-linked 6% agarose |
| packaging | pack of 5 × 5 mL |
| parameter | 42 psi (H2O at room temperature.) |
| working range | 3-12 |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Application: | IMAC Sepharose™ HP is supplied free of metal ions. It is charged by the user with the transition metal ion of choice (e.g. Cu2+, Zn2+, Ni2+, or Co2+); these metal ions will bind to the covalently immobilized chelating ligand on the Sepharose. |
| Features and Benefits: | • For optimizing purification of histidine-tagged Proteins when Ni2+ is not the best choice of metal ion. • Conveniently charge with your metal of choice. • Small bead size for high-performance purification. • High binding capacity. |
| General description: | HiTrap® IMAC HP is prepacked with IMAC Sepharose™ High Performance. The medium is charged with the metalion of your choice for Immobilized Metal ion Affinity Chromatography (IMAC) and subsequent high-resolution purification of polyhistidine tagged proteins. |
| Legal Information: | HiTrap is a registered trademark of Cytiva |
| Legal Information: | IMAC Sepharose products, Ni Sepharose products and Fe Sepharose products These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries. |
| Legal Information: | Sepharose is a trademark of Cytiva |
| Other Notes: | The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used. |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Preparation Note: | Store at 4 to 30 °C (20% Ethanol) |
| Symbol |  GHS02 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | UN1170 - class 3 - PG 3 - Ethanol, solution |
| UNSPSC | 23151816 |