IMAC Sepharose™ High Performance
SIGMA/GE17-0920-07 - Cytiva 17-0920-07, pack of 100 mL
Synonym: FPLC column packing; IMAC matrix; affinity resin; immobilized metal affinity chromatography polymer; stationary phase; uncharged IMAC resin
Product Type: Chemical
| avg. part. size | 34 μm |
| capacity | ≥40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.) |
| cleaning in place | 2-14(Medium uncharged with metal ions.) |
| manufacturer/tradename | Cytiva 17-0920-07 |
| matrix | highly cross-linked 6% agarose |
| packaging | pack of 100 mL |
| working range | 3-12 |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Application: | Conveniently charge with your metal of choice. IMAC Sepharose™ FF is supplied free of metal ions. It is charged by the user with the transition metal ion of choice (e.g. Cu2?, Zn2?, Ni2?, or Co2?); these metal ions will bind to the covalently immobilized chelating ligand on the Sepharose. Small bead size for high performance purification and high binding capacity. |
| Features and Benefits: | • For optimizing purification of histidine-tagged Proteins when Ni2+ is not the best choice of metal ion • Conveniently charge with your metal of choice • 34 μm bead size for high performance purification • High binding capacity |
| General description: | IMAC Sepharose™ High Performance media is easily charged by the user with the metalion of choice for Immobilized Metal ion Affinity Chromatography (IMAC) and subsequent purification of polyhistidine tagged proteins. |
| Legal Information: | IMAC Sepharose products, Ni Sepharose products and Fe Sepharose products These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries. |
| Legal Information: | Sepharose is a trademark of Cytiva |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Preparation Note: | Store at 4 to 30 °C (20% Ethanol) |
| Symbol |  GHS02 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | NONH for all modes of transport |
| UNSPSC | 41106500 |