IMAC Sepharose™ 6 Fast Flow
SIGMA/GE17-0921-08 - Cytiva 17-0921-08, pack of 100 mL
Synonym: FPLC column packing; IMAC matrix; affinity resin; immobilized metal affinity chromatography polymer; stationary phase; uncharged IMAC resin
Product Type: Chemical
| average diameter | 90 μm |
| capacity | ~40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.) |
| cleaning in place | 2-14(Medium uncharged with metal ions.) |
| manufacturer/tradename | Cytiva 17-0921-08 |
| matrix | 6% cross-linked agarose |
| packaging | pack of 100 mL |
| particle size | 45-165 μm |
| suitability | suitable for bioprocess medium |
| working range | 3-12 |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Features and Benefits: | • Uncharged IMAC resin allowing possibility to charge with your metal ion of choice for optimized selectivity • Convenient purification of histidine-tagged proteins, also when Ni2+ is not the best choice of metal ion • As BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support. • Available in the convenient and time-saving prepacked HiTrap® and HiPrep™ formats. |
| General description: | IMAC Sepharose™ 6 Fast Flow is a BioProcess IMAC medium, designed for purifying proteins and peptides that have an affinity for metal ions, such as histidine-tagged proteins but also untagged recombinant or native proteins. IMAC Sepharose™ 6 Fast Flow is a member of the Cytiva range of IMAC chromatography media for capture and intermediate purification. IMAC Sepharose™ 6 Fast Flow is composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix. IMAC Sepharose™ 6 Fast Flow is supplied free of metal ions, allowing the user to charge it with the most appropriate metal ion for purification of a target protein. The coupling chemistry and the novel ligand is designed to give minimal ligand leakage while providing high capacity. IMAC Sepharose™ 6 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess media range, IMAC Sepharose™ 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support. |
| Legal Information: | HiPrep is a trademark of Cytiva |
| Legal Information: | HiTrap is a registered trademark of Cytiva |
| Legal Information: | IMAC Sepharose products, Ni Sepharose products and Fe Sepharose products These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries. |
| Legal Information: | Sepharose is a trademark of Cytiva |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Preparation Note: | Store at 4 to 30 °C (20% Ethanol) |
| Symbol |  GHS02 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | NONH for all modes of transport |
| UNSPSC | 41106500 |