rProtein A Sepharose™ Fast Flow
SIGMA/GE17-1279-02 - Cytiva 17-1279-02, pack of 25 mL
Synonym: affinity resin; chromatography resin; gel; mAb purification resin; matrix; protein A resin
Product Type: Chemical
| capacity | ~35 mg binding capacity (human lgG/ml medium) |
| cleaning in place | 3-12 |
| crosslinking | cross-linked |
| form | resin |
| manufacturer/tradename | Cytiva 17-1279-02 |
| matrix | 4% cross-linked agarose |
| matrix active group | Recombinant protein A (E. coli) |
| operating pH | 3-10 |
| packaging | pack of 25 mL |
| particle size | ~90 μm |
| separation technique | affinity |
| storage condition | (20% Ethanol) |
| storage temp. | 2-8°C |
| technique(s) | liquid chromatography (LC): suitable |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Features and Benefits: | • For monoclonal and polyclonal antibody purification at both laboratory and process scale. • Well established among Protein A resins as excellent for monoclonal antibody (mAb) processes. • Free from animal-derived components. • Hydrophilic base matrix ensures low levels of non-specific binding and low levels of host cell-derived impurities in the elution pool. • Fulfills industrial demands for security of supply, robust performance, and regulatory support. |
| General description: | rProtein A Sepharose 4 Fast Flow is an affinity resin for antibody purification at laboratory and process scale. Monoclonal and polyclonal antibody purification rProtein A Sepharose 4 Fast Flow is built upon the well-established Sepharose Fast Flow platform and is used in many approved monoclonal antibody processes. It is composed of recombinant protein A coupled to the cross-linked 4% agarose base matrix.The highly crosslinked agarose resin enables high flow rates and high productivity. Protein A resin characteristics The recombinant protein is produced in E. coli and engineered with an oriented coupling to enhance binding capacity. Epoxy-based coupling ensures low ligand leakage. Specificity of the recombinant protein for the Fc region of IgG is similar to the native protein, giving a high degree of selectivity and yield. Streamlined scale up Scale-up with this resin is straightforward and is further aided by a range of prepacked process development tools, making purification scale-up straightforward. |
| Legal Information: | Sepharose is a trademark of Cytiva |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Symbol |  GHS02 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | 2-8°C |
| UNSPSC | 41106500 |