HiTrap® rProtein A Fast Flow
SIGMA/GE17-5079-02 - Cytiva 17-5079-02, pack of 2 × 1 mL
Synonym: affinity column; chromatography column; mAb purification column; prepacked column; protein A column
Product Type: Chemical
HiTrap rProtein A FF
| bed volume | 1 mL |
| capacity | ~35 mg binding capacity (human lgG / ml medium) |
| cleaning | 3-12 |
| column I.D. × L | 7 mm × 25 mm |
| crosslinking | cross-linked |
| form | prepacked column |
| manufacturer/tradename | Cytiva 17-5079-02 |
| matrix | 4% cross-linked agarose |
| matrix active group | recombinant protein A (E. coli) |
| operating pH | 3-10 |
| packaging | pack of 2 × 1 mL |
| parameter | 0.5 Mpa max. pressure |
| 1 mL/min flow rate | |
| 4 mL/min max. flow rate | |
| particle size | ~90 μm |
| separation technique | affinity |
| storage condition | (20% Ethanol) |
| storage temp. | 2-8°C |
| technique(s) | liquid chromatography (LC): suitable |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Application: | The recombinant Protein A ligand (produced in E. coli) is coupled to Sepharose™ Fast FLow by a technique which generates a stable thioether linkage between rProtein A and the base matrix. The coupling technique is optimized to give high binding capacity for IgG. |
| Features and Benefits: | • Quick, convenient preparative purification of monoclonal antibodies. • Well-established Protein A-based medium used in many approved MAb processes. • Recombinant Protein A exhibits similar Fc region specificity to that of native Protein A but shows enhanced binding capacity and fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification. • Simple operations with a syringe, pump, or high-performance chromatography system, such as ÄKTA® design. |
| General description: | Capacity of HiTrap rProtein A for some monoclonal antibodies. Running conditions: Binding buffer: 20 mM sodium phosphate (incl. 3 M NaCl for IgG), pH 7.0. Elution buffer: 0.1 M sodium citrate, pH 3.0. |
| General description: | HiTrap® rProtein A FF columns are prepacked with rProtein A Sepharose™ Fast FLow for purification and fractionation of IgG subclasses and IgG from different sources, including cell supernatants and ascites. |
| General description: | pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH. |
| Legal Information: | ÄKTA is a registered trademark of Cytiva |
| Legal Information: | HiTrap is a registered trademark of Cytiva |
| Legal Information: | Sepharose is a trademark of Cytiva |
| Other Notes: | The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used. |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Symbol |  GHS02 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | UN1170 - class 3 - PG 3 - Ethanol, solution |
| Storage Temp. | 2-8°C |
| UNSPSC | 23151816 |