GSTrap™ Fast Flow
SIGMA/GE17-5131-02 - Cytiva 17-5131-02, pack of 5 × 5 mL
Synonym: GSTrap Column
Product Type: Chemical
GSTrap FF
| bed volume | 5 mL |
| capacity | >10 mg/mL binding capacity |
| cleaning | 3-12 |
| column I.D. × L | 16 mm × 25 mm |
| crosslinking | cross-linked |
| form | prepacked column |
| manufacturer/tradename | Cytiva 17-5131-02 |
| matrix | highly cross-linked 4% agarose |
| matrix active group | glutathione |
| operating pH | 3-12 |
| packaging | pack of 5 × 5 mL |
| parameter | 0.5 Mpa max. pressure |
| 1.5-2.5 mL/min flow rate (sample loading) | |
| 7 mL/min flow rate (wash and elution) | |
| particle size | ~90 μm |
| separation technique | affinity |
| storage condition | (20% ethanol) |
| storage temp. | 2-30°C |
| technique(s) | liquid chromatography (LC): suitable |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Features and Benefits: | • Fast and simple one-step purification of GST-tagged proteins. • Mild elution conditions preserving protein antigenicity and function. • Easy scale-up by connecting the columns in series. • Sepharose™ 4 Fast Flow provides good flow properties. |
| General description: | GSTrap™ FF columns are prepacked Glutathione Sepharose™ Fast Flow columns for fast, convenient, one-step purification of glutathione S-transferase (GST) tagged proteins. GST*-tagged proteins can be purified directly from pretreated bacterial lysates using GSTrap™ FF.GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves protein antigenicity and function. If desired, cleavage of the protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods. The medium, Glutathione Sepharose™ 4 Fast Flow, is also available as lab packages and is an excellent choice for scale-up.The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as AKTA design or FPLC™ System. * See the section for licensing information in our terms and conditions. |
| General description: | The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used. |
| Legal Information: | FPLC is a trademark of Cytiva |
| Legal Information: | GSTrap is a trademark of Cytiva |
| Legal Information: | Sepharose is a trademark of Cytiva |
| Other Notes: | The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used. |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Preparation Note: | Store at 4 to 30 °C (20% Ethanol) |
| Symbol |  GHS02 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | UN1170 - class 3 - PG 3 - Ethanol, solution |
| Storage Temp. | 2-30°C |
| UNSPSC | 23151817 |