Glutathione Sepharose™ 4 Fast Flow
SIGMA/GE17-5132-03 - Cytiva 17-5132-03, pack of 500 mL
Synonym: Glutathione Column; Sepharose 4 Fast Flow
Product Type: Chemical
| capacity | >10 mg/mL binding capacity |
| cleaning | 3-12 |
| crosslinking | cross-linked |
| form | resin |
| manufacturer/tradename | Cytiva 17-5132-03 |
| matrix | highly cross-linked 4% agarose |
| matrix active group | Glutathione |
| operating pH | 3-12 |
| packaging | pack of 500 mL |
| parameter | 100 cm/hr max. flow rate (sample loading) |
| 100-300 cm/hr flow rate | |
| particle size | ~90 μm |
| separation technique | affinity |
| storage condition | (20% ethanol) |
| storage temp. | 2-30°C |
| technique(s) | liquid chromatography (LC): suitable |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Features and Benefits: | • Fast, one-step purification of GST-tagged proteins • Mild elution conditions preserving protein antigenicity and function • For scale-up due to high flow properties • Glutathione coupled to Sepharose™ 4 Fast Flow via a 10-carbon spacer arm |
| General description: | Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution. |
| General description: | Glutathione Sepharose™ 4 Fast Flow, 500 mL Glutathione Sepharose™ 4 Fast Flow is an affinity medium for fast, one-step purification of Glutathione S-Transferase (GST) tagged proteins. Provides good flow properties, excellent for scale-up. Glutathione Sepharose™ 4 Fast Flow is used for the purification of GST-tagged proteins from bacterial lysates and other S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function. Sepharose™ 4 Fast Flow provides good flow properties and is excellent for scaling up. |
| Legal Information: | Sepharose is a trademark of Cytiva |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Preparation Note: | Store at 4 to 30 °C (20% Ethanol) |
| Symbol |  GHS02 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | 2-30°C |
| UNSPSC | 41106500 |