HiTrap® Benzamidine Fast Flow
SIGMA/GE17-5143-02 - Cytiva 17-5143-02, pack of 2 × 1 mL
Synonym: chromatography column; cleanup chromatography column; factor Xa affinity column; p-aminobenzamidine column; prepacked column; thrombin affinity column
Product Type: Chemical
HiTrap Benzamidine FF (High Sub), 2 x 1 mL
| average diameter | 90 μm |
| bed size | 7 mm × 25 mm |
| bed volume | 1 mL |
| cleaning | 1-9 |
| column I.D. | 7 mm |
| ligand | p-aminobenzamidine |
| manufacturer/tradename | Cytiva 17-5143-02 |
| matrix | highly cross-linked 4% agarose |
| packaging | pack of 2 × 1 mL |
| parameter | 42 psi |
| storage condition | (0.05 M Sodium Acetate, pH 4.0 in 20% Ethanol) |
| storage temp. | 2-8°C |
| working range | 2-8 |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Application: | HiTrap® Benzamidine FF (high sub) are prepacked 1 mL and 5 mL columns for convenient, one-step removal and/or purification of trypsin, trypsin-like serine proteases, and zymogens including urokinase and prekallikrein. Removal of serine proteases is easily done directly from serum, monoclonal cell supernatants and bacterial lysates. The medium, Benzamidine Sepharose™ 4 Fast FLow (high sub), is also available as lab packages and is an excellent choice for scale-up. The columns can be operated using syringe, peristaltic pump or liquid chromatography system such as ÄKTAdesign or FPLC™ System. |
| Features and Benefits: | • Effective removal of thrombin and factor Xa after tag cleavage of recombinant Proteins. • Removal of serine proteases is easily done directly from serum, monoclonal cell supernatants and bacterial lysates. • HiTrap® Benzamidine FF for convenient, one-step removal and/or purification of trypsin, trypsin-like serine proteases, and zymogens including urokinase and prekallikrein. • High binding capacity. • The columns can be operated using syringe, peristaltic pump or liquid chromatography system such as ÄKTA® design or FPLC™ System. |
| General description: | HiTrap® Benzamidine FF (high sub) are prepacked with Benzamidine Sepharose™ 4 Fast FLow (high sub) and designed specifically for the removal and/or purification of serine proteases such as thrombin. |
| Legal Information: | ÄKTA is a registered trademark of Cytiva |
| Legal Information: | FPLC is a trademark of Cytiva |
| Legal Information: | HiTrap is a registered trademark of Cytiva |
| Legal Information: | Sepharose is a trademark of Cytiva |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Symbol |  GHS02 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | UN1170 - class 3 - PG 3 - Ethanol, solution |
| Storage Temp. | 2-8°C |
| UNSPSC | 23151816 |