Ni Sepharose™ High Performance
SIGMA/GE17-5268-02 - Cytiva 17-5268-02, pack of 100 mL
Synonym: High Performance Ni Resin; Ni Sepharose
Product Type: Chemical
| capacity | ≥40 mg binding capacity (histidine-tagged protein / mL medium) |
| cleaning in place | 2-14(Ni2+ stripped medium.) |
| crosslinking | cross-linked |
| form | resin |
| manufacturer/tradename | Cytiva 17-5268-02 |
| matrix | highly cross-linked 6% agarose |
| matrix active group | Nickel |
| operating pH | 3-12(Ni²⁺ stripped medium.) |
| packaging | pack of 100 mL |
| parameter | 150 cm/hr max. flow rate |
| particle size | ~34 μm |
| separation technique | affinity |
| storage condition | (20% ethanol) |
| storage temp. | 2-30°C |
| technique(s) | liquid chromatography (LC): suitable |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Features and Benefits: | • High-performance purification of histidine-tagged proteins • Negligible leakage of Ni2+ ions. • High binding capacity, at least 40 mg/mL medium • Compatible with a wide range of reducing agents, detergents, denaturants and other additives • 34 μm bead size for improved resolution |
| General description: | Ni Sepharose™ High Performance affinity media for high-resolution, high capacity purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC). Ni Sepharose™ High Performance consists of highly cross-linked agarose beads to which a chelating group has been coupled. This chelating group is precharged with Ni2+, which will selectively retain proteins with exposed histidine groups. The high stability and broad compatibility of Ni Sepharose™ High Performance maintains biological activity and increases product yield, at the same time as it greatly expands the range of suitable operating conditions. |
| Legal Information: | IMAC Sepharose products, Ni Sepharose products and Fe Sepharose products These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries. |
| Legal Information: | Sepharose is a trademark of Cytiva |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Preparation Note: | Store at 4 to 30 °C (20% Ethanol) |
| Symbol |  GHS09 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | 2-30°C |
| UNSPSC | 41106500 |