HisTrap™ Fast Flow Crude
SIGMA/GE17-5286-01 - Cytiva 17-5286-01, pack of 5 × 5 mL
Synonym: Histidine-tagged liquid chromatography column, FF crude columns, IMAC columns, protein purification columns
Product Type: Chemical
HisTrap FF Crude
| average diameter | 90 μm |
| bed volume | 5 mL |
| capacity | ~40 mg binding capacity (histidine tagged protein / mL medium) |
| cleaning | 2-14(Ni2+-stripped medium.) |
| column I.D. × L | 16 mm × 25 mm |
| crosslinking | cross-linked |
| form | prepacked column |
| manufacturer/tradename | Cytiva 17-5286-01 |
| matrix | 6% cross-linked agarose |
| matrix active group | Ni2+ ions |
| packaging | pack of 5 × 5 mL |
| parameter | 20 mL/min max. flow rate |
| 5 bar max. pressure | |
| 5 mL/min flow rate | |
| particle size | 45-165 μm |
| separation technique | affinity |
| shipped in | ambient |
| storage condition | (20% Ethanol) |
| storage temp. | 2-30°C |
| technique(s) | liquid chromatography (LC): suitable |
| working range | 3-12(Ni2+-stripped medium.) |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Features and Benefits: | • Optimized for purification of histidine-tagged proteins directly from homogenized, unclarified cell lysates. • High binding capacity, approx. 40 mg/mL medium and negligible Ni2+ leakage • Compatible with a wide range of reducing agents, detergents, denaturants, and other additives. • Reduced sample preparation time, which minimizes risk of protein degradation by proteases. • No need for centrifugation or filtration of the samples. Direct sample application. • Reliable purification of histidine-tagged proteins directly from unclarified lysates–simply sonicate and run. • Reduced sample preparation time, which minimizes risk of protein degradation by proteases • Simple operation with a syringe, pump, or chromatography system such as AKTAdesign or FPLC™ System • Permit rapid yet reliable separations with a minimum of sample preparation and equipment |
| General description: | HisTrap™ FF crude columns offer the convenience of simple, one-step purification of histidine-tagged proteins directly from homogenized, unclarified cell lysate by immobilized metal ion affinity chromatography (IMAC). HisTrap™ FF crude is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow. This prepacked column is intended for purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC). After thorough cell disruption, it is possible to load the unclarified lysate on the column without precentrifugation and filtration of the sample. Extending the duration of the mechanical treatment of the sample to ensure a more complete lysis is recommended. For optimal results, we recommend first addition of lysozyme and DNase I followed by a mechanical lysis, for example by sonication. The homogenized sample can then be loaded directly on the column without a clarification step which may prevent degradation of the target protein and increase the activity. Ni Sepharose™ 6 Fast Flow has low nickel ion (Ni2+) leakage and is compatible with a wide range of additives used in protein purification. The special design of the column in combination with the medium, provide fast, simple, and convenient purifications. Short purification time generally minimizes deleterious effects, such as degradation and oxidation of sensitive target proteins, and is therefore of great importance. HisTrap™ FF crude columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as AKTA design chromatography systems. |
| Legal Information: | FPLC is a trademark of Cytiva |
| Legal Information: | HisTrap is a trademark of Cytiva |
| Legal Information: | Sepharose is a trademark of Cytiva |
| Preparation Note: | Storage Temp.: 4°C to 30°C |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Preparation Note: | Store at 4 to 30 °C (20% Ethanol) |
| Symbol |  GHS09 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | UN1170 - class 3 - PG 3 - Ethanol, solution |
| Storage Temp. | 2-30°C |
| UNSPSC | 23151816 |